Received: 14-02-2023
Accepted: 07-04-2023
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Cloning and Sequencing of Genes Encoding Malonyl-CoA Synthetase (matB) and Malonyl-CoA Decarboxylase (matC) from Rhizobium leguminosarumKTCC 2362
,
Nguyen Quang Duc Tien
1
,
Nguyen Hoang Loc
1
,
Nguyen Huy Thuan
2
Abstract
matB(malonyl-CoA synthetase) and matC(malonyl CoA decarboxylase) are two enzymes involved in the malonyl-CoA metabolism, an essential precursor in metabolic processes in prokaryotes and eukaryotes such as nitrogen fixation, fatty acid synthesis, secondary metabolites, brain development, etc. Therefore, the study's objective was to clone the genes matBand matCthat can be used in the biosynthesis of the bioactive compound using malonyl-CoA as a precursor. matBandmatCwere isolated by PCR and ligated to cloning vector pGEM®-T Easy and sequenced. The obtained sequences were analyzed and compared to the reference sequence (AF117694.1) in the Genebank. In conclusion, Rhizobium leguminosarumKTCC 2362 - derived matBand matCgenes were cloned and sequenced successfully.
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