Detection and Identification of Pinworm Syphacia obvelatain Experimental Mice

Received: 13-12-2023

Accepted: 23-05-2024

DOI:

Views

10

Downloads

117

Issue: Vol. 22 No. 5 (2024)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Yen, N., & Hieu, N. (2024). Detection and Identification of Pinworm Syphacia obvelatain Experimental Mice. Vietnam Journal of Agricultural Sciences, 22(5), 631–638. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1317

Detection and Identification of Pinworm Syphacia obvelatain Experimental Mice

Nguyen Thi Hoang Yen (*) 1, 2 , Nguyen Chi Hieu 3

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Bộ môn Ký sinh trùng, Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 3 Viện Kiểm định Quốc gia Vắc xin và Sinh phẩm y tế

    • Abstract


    This study was conducted to identify the species of pinworms in experimental mice. Eggs of pinworms were examined from four white mice that were purchased from an animal facility before the conduct of experimental infection. The eggs were detected by the perianal tape test method, adult worms were collected by necropsy method, and then eggs and adult worms were identified based on their morphology. Besides, PCR and sequencing techniques were also applied to confirm the species identification. The results show that eggs were pointed oval (with one side flatted) in shape and approximately 129.2 x 39.17m in size. Adult worms were 3-5mm in length, having two subtle cervical alea, a bulb at the end of the esophagus, and a long and pointed tail. PCR and sequencing techniques revealed the species of pinworm to be Synphacia obvelata. Even though the scope of the study was still limited, the results of the study provided information on the presence of S. obvelatain experimental mice and gave a warning signal about monitoring and eradication of pinworms for experimental animal facilities.

    References

    Agerborg S.S., Garza K.M. & Tung K.S. (2001). Intestinal parasitism terminates self tolerance and enhances neonatal induction of autoimmune disease and memory. European Journal of Immunology. 31: 851-859.

    Baker D. (1998). Natural pathogens of laboratory mice, rats, and rabbits and their effects on research. Clinical Microbiology Review. 11: 231-266.

    Bazzano T., Restel T.I., Pinto R.M. & Gomes D.C. (2002). Patterns of infection with nematodes Syphacia obvelata and Aspicularis tetraptera in conventionally maintained laboratory mice. Memórias do Instituto Oswaldo Cruz. 97: 847-853.

    Beattie G.M., Baird S., Lannom R., Slimmer S., Jensen F.C. & Kaplan N.O. (1980). Induction of lymphoma in athymic mice: a model for study of the human disease. Proceedings of the National Academy of Sciences. 77: 4971-4974.

    Behnke J.M., Lewis J.W., Zain S.N. & Gilbert F.S. (1999). Helminth infections in Apodemus sylvaticus in southern England: interactive effects of host age, sex and year on the prevalence and abundance of infections. Journal of Helminthology. 73: 31-44.

    Chan K.F. (1952). Life cycle studies on the nematode Syphacia obvelata. American Journal of Hygiene. 56: 14-21.

    Chan K.F. (1955). The distribution of larval stages of Aspiculuris tetraptera in the intestine of mice. Journal of Parasitology. 41: 529-532.

    Chen X.M., Li X., Lin R.Q., Deng J.U., Fan W.Y., Yuan Z.G., Liao M. & Zhu X.Q. (2011). Pinworm infection in laboratory mice in southern China. Laboratory Animals. 45: 58-60.

    Derothe J.M., Loubes C., Orth A., Renaud F., & Moulia C. (1997). Comparison between patterns of pinworm infection (Aspiculuris tetraptera) in wild and laboratory strain of mice, Mus musculus. International Journal of Parasitology. 27: 645-651.

    Faust E.C. & Russell P.F. (1964). Craig and Faust’s clinical parasitology. 7th Edition. Lea & Febiger, Philadelphia.

    Fiette L. & Slaoui M.(2011). Necropsy and Sampling Procedures in Rodents. Jean-Charles Gautier (ed.), Drug Safety Evaluation: Methods and Protocols, Methods in Molecular Biology. 691: 39-67.

    Flynn R. (1973). Nematodes. In parasites of laboratory animals. Iowa State University Press, Ames. pp. 203-320

    Grencis R.K. (1997). Th2-mediated host protective immunity to intestinal nematode infections. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. 352: 1377-1384.

    Jacoby R.O. & Linsey J.R. (1998). Risks of infection among laboratory rats and mice at major biomedical research institutions. ILAR journal. 39: 266-271.

    Lübcke R., Hutchenson F.A.R. & Barbezat G.O. (1992). Impaired intestinal electrolyte transport in rats infested with the common parasite Syphacia muris. Digestive Diseases and Sciences. 37: 60-64.

    Malsawmtluangi C. & Tandon V. (2009). Helminth parasite spectrum in rodent hosts from bamboo growing areas of Mizoram, North-east India. 33: 28-35.

    Marx M.B. (1991). Parasites, pets, and people. Prime Care. 18: 153-165.

    Michels C., Goyal P., Nieuwenhuizen N. & Brombacher F. (2006). Infection with Syphacia obvelata (Pinworm) induces protective Th2 immune responses and influences ovalbumin-induced allergic reactions. Infection and Immunity. 74: 5926-32.

    Milazzo C., Cagnin M., Dibella C., Geraci F. & Ribas A. (2010). Helminth fauna of commensal rodents, Mus musculus (Linnaeus, 1758) and Rattus rattus (Linnaeus, 1758) (Rodentia, Muridae) in Sicily (Italy). Revista Ibero-Latinoamericana de Parasitología. 69: 194-98.

    Nguyen Y.T.H., Wang Z.Z., Maruyama H., Horii, Y., Nonaka N., Yoshida A., 2016. Evaluation of realtime PCR assay for the detection of Ascaris suum contamination in meat and organ meats. J. Food Saf. 37(2), 1-6.

    Ooi H.K., Oku Y. & Kamiya M. (1994). Aspiculuris tetraptera. In: Waggie, K., Kagiyama, N., Allen, A., Nomura, T. (Eds.), Manual of Microbiologic Monitoring of Laboratory Animals. seconded. National Institutes of Health, United States of America, pp. 173-175.

    Pakdel N., Naem S., Rezaei F. & Chalehchaleh A. (2013). A survey on helminthic infection in mice (Mus musculus) and rats (Rattus norvegicus and Rattus rattus) in Kermanshah. Iran Veterinary Research Forum. 4: 105-09.

    Parel J.D., Galula J.U. & Ooi H.K. (2008). Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris and Aspiculuris tetraptera and development of a PCR-based method for identification. Veterinary Parasitology. 153: 379- 83.

    Pearson D.J. & Taylor G. (1975). The influence of the nematode Syphacia obvelata on adjuvant arthritis in rats. Immunology. 29: 391-396.

    Pinto R.M., Goncalves L., Noronha D., Gomes D.C. (2001). Worm burdens in outbred and inbred laboratory rats with morphometric data on Syphacia muris (Yamaguti, 1935) Yamaguti, 1941 (Nematoda Oxyuroidea). Memórias do Instituto Oswaldo Cruz. 96: 133-136.

    Pisanu B., Chapuis J.L. & Durette-Desset M.C. (2001). Helminths from introduced small mammals on Kerguelen, Crozet, and Amsterdam Islands (southern Indian Ocean). Journal of Parasitology. 87: 1205-1208.

    Pritchett K.R. (2007). Helminth parasites of laboratory mice. The mouse in biomedical research, 2nd Edition. History, Wild Mice, and Genetics. American College of Laboratory Animal Medicine. 2: 55-564.

    Reuter J.D. & Dysko R.C. (2003). Quality assurance/surveillance monitoring programs for rodent colonies, pp 1-14. Laboratory Animal Medicine and Management. (Eds) Reute J.D. and Suckow M.A. International Veterinary Information Service, Ithaca, New York.

    Sato Y., Ooi H.K., Nonaka N., Oku Y. & Kamiya M. (1995). Antibody production in Syphacia obvelata infected mice. Journal of Parasitology. 81: 559-562.

    Singleton G.R., Smith A.L., Shellam G.R., Fitzgerald N. & Muller W.J. (1993). Prevalence of viral antibodies and helminths in field populations of house mice (Mus domesticus) in southeastern Australia. Epidemiology & Infection. 110: 399-417.

    Stewart A., Lowe A., Smales L., Bajer A., Radley B., Dwużnik D., Franssen F., Griffith J., Stuart P., Turner C., Zaleśny G. & Behnke J.M. (2017). Parasitic nematodes of the genus Syphacia Seurat, 1916 infecting Muridae in the British Isles, and the peculiar case of Syphacia frederici. Parasitology. 143(3): 1-12.

    Stone W.B. & Manwell R.D. (1966). Potential helminth infections in humans from pet or laboratory mice and hamsters. Public Health Reports. 81(7): 647-653.

    Sundar S.T.B., Harikrishnan T.J., Latha B.R., Chandra G.S., Kumar T.M.A.S., Ramesh S., Srinivasan M.R., Azhahianambi P., Gomathinayagam S., Raman M., Tirumurugaan K.G. & Pandian S.S. (2008). Prevalence of syphaciosis in laboratory rodents with emphasis on species identification, treatment and Th2 cytokine response. Indian Journal of Animal Sciences. 88(7): 765-770.

    Wagner M. (1988). The effect of infection with the pinworm (Syphacia muris) on rat growth. Laboratory Animal Science. 38: 476-478.

    Zenner L. (1998). Effective eradication of pinworms (Syphacia muris, Syphacia obvelata and Aspiculuris tetraptera) from a rodent breeding colony by oral anthelmintic therapy. Laboratory Animals. 32: 337-342.