Received: 22-12-2023
Accepted: 12-04-2024
DOI:
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Biological and Molecular Biological Characterizations ofa Pseudorabies Virus Strain Isolated from Pigs in Vietnam
,
Nguyen Ba Hien
2
,
HuynhThi My Le
1
,
Dang Huu Anh
1
,
Le Van Truong
1
,
Vu Thi Ngoc
1
,
Cao Thi Bich Phuong
1
,
Le Huynh Thanh Phuong
3
Keywords
Pseudorabies virus, pig, biology, molecular biology
Abstract
Pseudorabies has been known in Vietnam for a long time and is still one of the most devastating diseases of particular concern of animal breeders. Despite the emerging and prevailing of variants the virus in many countries around the world since 2010, to date, there is scarce information about the disease as well as the virus circulating in Vietnam. This study aimed to clarify some basic biological and molecular characteristics of an isolate, PRV.PT.001 strain. The isolated strain quickly adapted, induced typical syncytium on Vero cells and reached a relatively high titer after 7 passages (106.58TCID50/ml). The isolate had common characteristics of the virus that causes pseudorabies: it is pathogenic to rabbits and piglets, induces consistent lesions in the respiratory tract, and is widely distributed in many internal organs. Regarding genetic characteristics, strain PRV.PT.001 belongs to genotype 2, carrying typical insertion-deletion mutations in 3/4 of the investigated surface glycoproteins (gB, gC, gD ); but has the characteristic insertion-deletion mutation of genotype 1 in gE glycoprotein.
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