Study on Sequences of Gene Encoding Lea Protein in Some Salt Resistant Rice Varieties

Received: 13-05-2014

Accepted: 15-07-2014

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Issue: Vol. 12 No. 4 (2014)

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NÔNG HỌC

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An, T., Nghiem, D., Hanh, T., Cuong, P., & Phuc, D. (2024). Study on Sequences of Gene Encoding Lea Protein in Some Salt Resistant Rice Varieties. Vietnam Journal of Agricultural Sciences, 12(4), 516–521. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/127

Study on Sequences of Gene Encoding Lea Protein in Some Salt Resistant Rice Varieties

Tran Xuan An (*) 1 , Dang Xuan Nghiem 2 , Tang Thi Hanh 3 , Pham Van Cuong 3 , Do Thi Phuc 1

  • 1 Khoa Sinh học, Trường Đại học Khoa học Tự nhiên, Đại học Quốc gia Hà Nội
  • 2 Khoa Công nghệ Sinh học, Học viện Nông nghiệp Việt Nam
  • 3 Khoa Nông học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Abiotic stress tolerance, LEA protein, Lea21, rice, salinity resistance

    Abstract


    LEA protein comprises a large group of proteins which highly accumulates in plant seeds at late stage of maturation. LEA protein is proven to be involved in abiotic stress responses in plants, such as under dehydration and salinity. Genes encoding LEA proteins are divided into 6 different groups. Lea21 gene belongs to group 5. Up to now, there is no report about sequence polymorphsim of Lea21 gene in Vietnamese rice varieties. Therefore, in order to investigate the genetic diversity in rice, Lea21 gene from some salt resistant rice varieties was amplified and sequenced. The Lea21 gene, which consists of 2 exon and 1 intron, was sucessfully amplified using PCR technique and was sequenced.. It was shown that no difference in nucleotide sequences among investigated rice varities was observed, indicating that the sequence of Lea21 gene is highly conserved.

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