Received: 21-08-2023
Accepted: 05-01-2024
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Expression of FIT Protein in Response to Different Iron(Fe) Levels and Oxidative Stress in Arabidopsis
,
Vu Thi Thuy Hang
2
,
Phan Thi Thu Hien
3
Keywords
Arabidopsis, FIT, FIT overexpression, protein quantification, Fe level, oxidative sress
Abstract
This study aimed to evaluate the expression of FIT (FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) at translational level under different iron (Fe) conditions prolonged 8 and 10 days associated with oxidative stress. This study assessed the expression of FIT at the translational level using the Western Blot technique in transgenic Arabidopsis plants overexpressing FIT for 8 and 10 days. FIT protein contents will be quantified using ImageJ software. The results showed that under Fe deficiency and excess conditions, the expression of FIT protein in 10-day-old plants was lower than in 8-day-old plants, in contrast to Fe-sufficient conditions. Under oxidative stress conditions, the expression of FIT increased except in the condition of Fe deficiency for 8 days. This result helps to complement the further study of the mechanism of FIT under conditions containing different Fe content.
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