Study on Preparation of Chitosan Nanocapsules Transduced with Trans- cinnamaldehyde for Communication Inhibition in E. coli Top10

Received: 27-02-2023

Accepted: 05-01-2024

DOI:

Views

0

Downloads

0

Issue: Vol. 22 No. 1 (2024)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Gioi, D., Hao, N., & Luu, N. (2024). Study on Preparation of Chitosan Nanocapsules Transduced with Trans- cinnamaldehyde for Communication Inhibition in E. coli Top10. Vietnam Journal of Agricultural Sciences, 22(1), 115–124. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1249

Study on Preparation of Chitosan Nanocapsules Transduced with Trans- cinnamaldehyde for Communication Inhibition in E. coli Top10

Dong Huy Gioi (*) 1 , Nguyen Thanh Hao 1 , Nguyen Thi Bich Luu 1

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Chitosan, nanocapsules, nanoemulsion, bacteria, E. coli

    Abstract


    This study was conducted with the aim of preparing chitosan nanocapsules transduced with trans-cinnamaldehyde for communication inhibition in bacteria. We used emulsification method to synthesize nanocapsule and nanoemulsion complexes. The nanocapsule and nanoemulsion complexes were transduced with trans-cinnamaldehyde using an oil nucleus and chitosan as cover. The nanocomplex had a uniform particle structure, low dispersion, and the size ranges from 120 to 150nm. Both nanocapsule and nanoemulsion were stable in bacterial culture after 24h of incubation. The trans-cinnamaldehyde transduction efficiency for the nanocapsule and nanoemulsion complexes was 86 and 73%, respectively. Both complexes released the drug through two stages, slowly in the first 6 hours and followed by continuous drug release after 12 hours, reaching 35 and 45%, respectively, for the nanocapsule and nanoemulsion. Bioassays with the E. coli Top 10 biosensor showed that nanocapsule was superior to nanoemulsions in inhibiting bacterial communication, also known as quorum sensing. In the best formulation, nanocapsule inhibited the communication process in this bacterium up to 65%.

    References

    Antunes L.C.M., Ferreira R.B.R., Buckner M.M.C. & Finlay B.B. (2010). Quorum sensing in bacterial virulence. Microbiology. 156(8): 2271-2282. doi.org/10.1099/MIC.0.038794-0/cite/refworks.

    Brackman G., Defoirdt T., Miyamoto C., Bossier P., Calenbergh S. Van, Nelis H. & Coenye T. (2008). Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in Vibrio spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR. BMC Microbiology. 8(8): 1-14. doi.org/10.1186/1471-2180-8-149.

    Calvo P. & Remunan-Lopez C. (1997). Development of positively charged colloidal drug carriers: chitosan-coated polyester nanocapsules and submicron-emulsions. Colloid and Polymer Science. 275(1): 46-53. doi.org/10.1007/s003960050050.

    Chang C., Krishnan T., Wang H., Chen Y., Yin W., Chong Y., Tan L.Y., Chong T.M. & Chan K.G. (2014). Non-antibiotic quorum sensing inhibitors acting against N-acyl homoserine lactone synthase as druggable target. Scientific Reports. 4: 7245. doi.org/10.1038/srep07245.

    Devlieghere F., Vermeulen A. & Debevere J. (2004). Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiology. 21(6): 703-714. doi.org/10.1016/J.FM.2004.02.008

    Eberhard’, A. (1972). Inhibition and Activation of Bacterial Luciferase Synthesis. Journal of Bacteriology. 109(3): 1101-1105.

    Goycoolea F.M., Valle-Gallego A., Stefani R., Menchicchi B., David L., Rochas C., Santander-Ortega M.J. & Alonso M.J. (2012). Chitosan-based nanocapsules: Physical characterization, stability in biological media and capsaicin encapsulation. Colloid and Polymer Science. 290(14): 1423-1434. doi.org/10.1007/s00396-012-2669-z.

    Helander I.M., Nurmiaho-Lassila E.L., Ahvenainen R., Rhoades J. & Roller S. (2001). Chitosan disrupts the barrier properties of the outer membrane of Gram-negative bacteria. International Journal of Food Microbiology. 71(2-3): 235-244. doi.org/10.1016/S0168-1605(01)00609-2.

    Kaiser M., Pereira S., Pohl L., Ketelhut S., Kemper B., Gorzelanny C., Galla H.-., Moerschbacher, B.M. & Goycoolea F.M. (2015). Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells. Scientific Reports. 5: 10048. doi.org/10.1038/srep10048.

    Lam S.J., O’Brien-Simpson N.M., Pantarat N., Sulistio A., Wong E.H.H., Chen Y.Y., Lenzo J.C., Holden J.A., Blencowe A., Reynolds E.C. & Qiao G.G. (2016). Combating multidrug-resistant Gram-negative bacteria with structurally nanoengineered antimicrobial peptide polymers. Nature Microbiology. doi.org/10.1038/nmicrobiol.2016.162.

    Mancuso G., Midiri A., Gerace E. & Biondo C. (2021). Bacterial antibiotic resistance: the most critical pathogens. Pathogens. 10(10). doi.org/10.3390/pathogens10101310/S1.

    O’Callaghan K.A.M. & Kerry J.P. (2016). Preparation of low- and medium-molecular weight chitosan nanoparticles and their antimicrobial evaluation against a panel of microorganisms, including cheese-derived cultures. Food Control. 69: 256-261. doi.org/10.1016/J.FOODCONT.2016.05.005.

    Omwenga E.O., Hensel A., Shitandi A. & Goycoolea F.M. (2018). Chitosan nanoencapsulation of flavonoids enhances their quorum sensing and biofilm formation inhibitory activities against an E.coliTop 10 biosensor. Colloids and Surfaces B: Biointerfaces. 164: 125-133. doi.org/10.1016/j.colsurfb.2018.01.019.

    Perinelli D.R., Fagioli L., Campana R., Lam J.K.W., Baffone W., Palmieri G.F., Casettari L. & Bonacucina G. (2018). Chitosan-based nanosystems and their exploited antimicrobial activity. European Journal of Pharmaceutical Sciences. 117: 8-20. doi.org/10.1016/J.EJPS.2018.01.046.

    Qin X., Engwer C., Desai S., Vila-Sanjurjo C. & Goycoolea F.M. (2017). An investigation of the interactions between an E. colibacterial quorum sensing biosensor and chitosan-based nanocapsules. Colloids and Surfaces B: Biointerfaces. 149: 358-368. doi.org/10.1016/j.colsurfb.2016.10.031.

    Santander-Ortega M.J., Peula-García J.M., Goycoolea F.M. & Ortega-Vinuesa J.L. (2011). Chitosan nanocapsules: Effect of chitosan molecular weight and acetylation degree on electrokinetic behaviour and colloidal stability. Colloids and Surfaces B: Biointerfaces. 82: 571-580. doi.org/10.1016/j.colsurfb.2010.10.019.

    Sneader W. (2001). History of Sulfonamides. ELS. doi.org/10.1038/NPG.ELS.0003625.

    Tang K. & Zhang X.H. (2014). Quorum quenching agents: Resources for antivirulence therapy. Marine Drugs. 12(6): 3245-3282. doi.org/10.3390/md12063245.

    Thanh Nguyen H. & Goycoolea F.M. (2017). Chitosan/Cyclodextrin/TPP nanoparticles Loadedwith Quercetin as Novel Bacterial Quorum Sensing Inhibitors. Molecules (Basel, Switzerland). 22(11): 1975. doi.org/10.3390/molecules22111975.