Study on Identification and Culture Conditionfor Biosynthesis of Laccase by Trametes polyzona TĐ16

Received: 18-08-2023

Accepted: 26-12-2023

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Issue: Vol. 21 No. 12 (2023)

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NÔNG HỌC

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Lien, N., Huong, T., Hieu, N., Nhung, D., & Thao, P. (2024). Study on Identification and Culture Conditionfor Biosynthesis of Laccase by Trametes polyzona TĐ16. Vietnam Journal of Agricultural Sciences, 21(12), 1561–1568. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1227

Study on Identification and Culture Conditionfor Biosynthesis of Laccase by Trametes polyzona TĐ16

Nguyen Thi Hong Lien (*) 1 , Tran Thi Huong 1 , Nguyen Van Hieu 1 , Dang Thi Nhung 1 , Phan Thi Hong Thao 1

  • 1 Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam

    • Keywords

    Trametes polyzona, cultivation condition, identification, ITS-rDNA, laccase

    Abstract


    White-rot fungi are capble of degrading wood lignin by extracellular lignin modifying enzyme system, in which laccase plays an important role in ligninolysis and is widely used in some industries. This study was carried out with the objective of identifying the white rot fungus TD16 and selected suitable conditions for the biosynthesis of laccase to apply in the biopulping. The strain TD16 was studied for biological characteristics and sequence of the ITS - rDNA gene analyzed. The fruiting bodies have a scalloped shape and the edges are smooth. The upper surface of fruiting bodies is dark brown with concentric circular veins. The hymenium layer is white-brown and rough. On MEA medium, the colonies of TD16 fungal strain are fluffy, snow white, and evenly circular. The strain TD16 has a relatively fast growth rate and can produce extracellular enzymes. The strain TD16 was identified as Trametes polyzonaand named as Trametes polyzonaTD16. The optimal culture conditions for enhanced laccase biosynthesis were investigated. Trametes polyzonaTĐ16 showed the highest laccase activity of upto 8.135 U/ml on MT2 medium containing 0.0625mM CuSO4after 7 days of fermentation at 37ºC and pH of 5.0.

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