RAPD and ISSR Markers Assessment of Genetic Diversity of 12 Cordyceps Accessions collected in Vietnam

Received: 15-08-2023

Accepted: 25-12-2023

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Issue: Vol. 21 No. 12 (2023)

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NÔNG HỌC

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Hue, N., Thao, T., Linh, L., Thao, N., Nghien, N., & Son, D. (2024). RAPD and ISSR Markers Assessment of Genetic Diversity of 12 Cordyceps Accessions collected in Vietnam. Vietnam Journal of Agricultural Sciences, 21(12), 1549–1560. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1226

RAPD and ISSR Markers Assessment of Genetic Diversity of 12 Cordyceps Accessions collected in Vietnam

Nong Thi Hue (*) 1 , Tran Thi Thao 1 , Le Phuong Linh 1 , Ninh Thi Thao 1 , Ngo Xuan Nghien 2, 1 , Dinh Truong Son 3, 1

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 2 Viện Nghiên cứu và Phát triển Nấm ăn, Nấm dược liệu, Học viện Nông nghiệp Việt Nam
  • 3 Viện Sinh học Nông nghiệp, Học viện Nông nghiệp Việt Nam

    • Keywords

    Cordyceps, Cordyceps militaris, genetic diversity, ISSR, RAPD

    Abstract


    Cordyceps spp. belong to the most valuable medicinal caterpillar fungi. However, consumers hardly know the origin and species of Cordycepsspp. commercialized on the market. This study aimed to identify 12 collected Cordyceps samples collected at various locations in Vietnam by ITS sequencing and evaluate the genetic diversity these sampless using RAPD and ISSR markers. 12 Cordyceps accessions were identified as Cordyceps militarisusingITS sequencing. 15 RAPD markers and 15 ISSR markers successfully detected 340 loci of which 291 were polymorphic and a total of 2092 scorable bands were obtained.The similarity coefficientof 12 Cordyceps spp. accessionsrangedfrom 0.568 to 0.841 when combined RAPD and ISSR markers, indicating high genetic diversity. At a genetic similarity of 0.7, the UPGMA dendrogram constructed based on RAPD and ISSR makers separated 12 Cordyceps accessions into three distinct clusters. The correlation analysis between the matrices of similarity coefficients using Mantel’s test revealed a strong correlation among matrices analyzed by RAPD, ISSR or pooled data. Our results could provide useful information for the exploitation and breeding of Cordyceps militarisaccessions.

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