Selection of Potential Bacterial Strain for Controlling Gummosis Disease in Citrus (Citrus sinensis)

Received: 02-04-2022

Accepted: 05-07-2022

DOI:

Views

0

Downloads

0

Issue: Vol. 20 No. 8 (2022)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Hien, P., Diep, B., Quynh, N., Tung, P., & Tam, D. (2024). Selection of Potential Bacterial Strain for Controlling Gummosis Disease in Citrus (Citrus sinensis). Vietnam Journal of Agricultural Sciences, 20(8), 1066–1075. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1034

Selection of Potential Bacterial Strain for Controlling Gummosis Disease in Citrus (Citrus sinensis)

Pham Hong Hien (*) 1 , Bach Thi Diep 2 , Nguyen Thi Chuc Quynh 2 , Phung Quang Tung 2 , Dang Thi Thanh Tam 3

  • 1 Ban Khoa học và Hợp tác quốc tế, Viện Khoa học Nông nghiệp Việt Nam
  • 2 Viện Bảo vệ thực vật
  • 3 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Antagonistic activity, gummosis, Phytophthora citrophthora, Phytophthora palmivora, Bacillus siamensis

    Abstract


    The research aimed to identify potential biocontrol agents in controlling Phytophthora spp. that causes gummosis disease of citrus (C. sinensis). Out of 38 strains isolated from the soil samples collected from Hoa Binh and Tien Giang provinces, the strain LHB15 showed strong antagonistic activity against P. citrophthora 18PMS and P. palmivora 17PMS. Analysis of morphological characteristics and 16S rRNA sequence, the strain LHB15 was identified as B. siamensis. The optimal condition for the growth of B. siamensis LHB15 was determined in LB at 37C and pH 7. Testing the antagonistic activity against P. citrophthora18PMS in greenhouse conditions showed a strong reduction in the presence of fungi on soil and citrus roots after 45 days of treatment. The study indicated that the B. siamensisLHB15 is a potential strain for controlling gummosis in citrus.

    References

    Barquero M., Terrón A., Velázquez E. & González-Andrés F. (2016). Biocontrol of Fusarium oxysporum f.sp. phaseoliand Phytophthora capsiciwith autochthonous endophytes in common bean and pepper in Castilla y León (Spain). Biological Nitrogen Fixation and Beneficial Plant-Microbe Interaction. Springer International Publishing. pp. 221-235.

    Drenth A. & Guest D. (2004). Diversity and Management of Phytophthora in Southeast Asia. ACIAR monograph. 114.

    Erwin D.C. & Ribeiro O.K. (1996). Phytophthora diseases worldwide.APS Press, American Phytopathological Society. St. Paul, MN. 562p.

    Gorai P.S., Ghosh R., Mandal S., Ghosh S., Chatterjee S., Gond S.K. & Mandal N.C. (2021). Bacillus siamensisCNE6- a multifaceted plant growth promoting endophyte of Cicer arietinumL. having broad spectrum antifungal activities and host colonizing potential. Microbiological Research.252: 126859.

    Graham J. & Feichtenberger E. (2015). Citrus phytophthora diseases: Management challenges and successes. Journal of Citrus Pathology.2.

    Jagtap G.P., Dhavale M. & Dey U. (2012). Evaluation of natural plant extracts, antagonists and fungicides in controlling root rot, collar rot, fruit (brown) rot and gummosis of citrus caused by Phytophthoraspp. in vitro. pp. 27-47.

    Jamali F., Sharifi-Tehrani A., Okhovvat M., Zakeri Z. & Saberi-Riseh R. (2004). Biological control of chickpea Fusarium wilt by antagonistic bacteria under greenhouse condition. Commun Agric Appl Biol Sci.69(4): 649-51.

    Mekonen M., Ayalew A., Weldetsadik K. & Awol S. (2015). Assessing and Measuring of Citrus gummosis (Phytophthoraspp.) in Major Citrus Growing Areas of Ethiopia. Journal of Horticulture.2: 154.

    Mi Y., Zhao X., Liu F., Sun C., Sun Z. & Liu L. (2021). Changes in soil quality, bacterial community and anti-pepper Phytophthora disease ability after combined application of straw and multifunctional composite bacterial strains. European Journal of Soil Biology.105: 103329.

    Mounde L.G., Ateka E.M., Kihurani A.W., Wasilwa L. & Thuranira E.G. (2009). Occurrence and distribution of citrus gummosis (Phytophthoraspp.) in Kenya. Journal of Horticultural Science.2.

    Nguyễn Lân Dũng (2014). Giáo trình vi sinh vật học, Nhà xuất bản Hà Nội. tr. 198-212.

    Park B.R., Son H.J., Park J.H., Kim E.S., Heo S.J., Youn H.R., Koo Y.M., Heo A.Y., Choi H.W., Sang M.K., Lee S.W., Choi S.H. & Hong J.K. (2021). Chemical Fungicides and Bacillus siamensisH30-3 against Fungal and Oomycete Pathogens Causing Soil-Borne Strawberry Diseases. The plant pathology journal.37(1): 79-85.

    Rajput N., Atiq M., Tariq H., Modassar W. & Hameed A. (2020). Citrus Gummosis: A Formidable Challenge to Citrus Industry: A Review. 16: 131-144.

    Saidi N., Kouki S., M’hiri F., Hajlaoui M.R., Mahrouk M., Ouzari H., Jedidi N. & Hassen A. (2009). Characterization and selection ofBacillussp. strains, effective biocontrol agents against Fusarium oxysporum f. sp.radicis-lycopersici, the causal agent of Fusarium crown and root rot in tomato. Annals of Microbiology.59(2): 191.

    Tổng cục Thống kê (2021). Thông cáo báo chí về tình hình kinh tế - xã hội quý IV và năm 2021. Truy cập từ https://www.gso.gov.vn/du-lieu-va-so-lieu-thong-ke/2021/12/thong-cao-bao-chi-ve-tinh-hinh-kinh-te-xa-hoi-quy-iv-va-nam-2021/ ngày 01/03/2022.

    Živković S., Stojanović S., Ivanović Ž., Veljko G., Popović T. & Balaž J. (2010). Screening of antagonistic activity of microorganisms against Colletotrichum acutatumand Colletotrichum gloeosporioides. Archives of Biological Sciences.62.