Isolation and Antibiotic Susceptibility of Flavobacterium columnare Infecting Grass Carp Cultured in Northern Vietnam

Received: 23-02-2022

Accepted: 05-04-2022

DOI:

Views

0

Downloads

0

Issue: Vol. 20 No. 6 (2022)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Hoai, T., Nhinh, D., Trinh, T., Hoa, D., Tuan, N., & Van, K. (2024). Isolation and Antibiotic Susceptibility of Flavobacterium columnare Infecting Grass Carp Cultured in Northern Vietnam. Vietnam Journal of Agricultural Sciences, 20(6), 732–740. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1003

Isolation and Antibiotic Susceptibility of Flavobacterium columnare Infecting Grass Carp Cultured in Northern Vietnam

Truong Dinh Hoai (*) 1, 2, 3 , Doan Thi Nhinh 3 , Tran Thi Trinh 3 , Dang Thi Hoa 3 , Nguyen Ngoc Tuan 3 , Kim Van Van 3

  • 1 Khoa Chăn nuôi và NTTS
  • 2 Faculty of Animal Science and Aquaculture, Vietnam National University of Agriculture
  • 3 Khoa Thủy sản, Học viện Nông nghiệp Việt Nam

    • Keywords

    Flavobacterium columnare, grass carp, antibiotic resistance

    Abstract


    This study was conducted to identify the pathogen F. columnarefrom diseased grass carp cultured in northern Vietnam and to examine the susceptibility of this bacterium to various common antibiotics used in aquaculture. A total of 103 grass carp samples suspected to be infected with F. columnare were collected and screened from 25 grass carp farms in Ha Noi, Hai Duong and Bac Ninh provinces. The grass carps infected with F. columnareexhibited the symptoms of decayed, white-grey gill filaments, rotten fins, and skin discoloration. From 67 yellow and rhizoid isolates recovered from the collected samples, 25 representative isolates (one per farm) were identified by the phenotypic tests and PCR assays. Grass carps (20-25g) challenged with F. columnareat the dose of 2 ×106 CFU/ml by immersion in 2h resulted in 100% mortality within 48-72 hpi. Theantibiotic test showed that high proportion of F. columnareisolates were susceptible to doxycycline, amoxicillin, florfenicol, and oxytetracycline (92-96%), but resistant to sulfamethoxazole/trimethoprim (44%) and trythromycin (16%). The present study provided necessary information for diagnosing and controlling F. columnarein grass carp.

    References

    Bernardet J.F. & Grimont P.A. (1989). Deoxyribonucleic acid relatedness and phenotypic characterization of Flexibacter columnarissp. nov., nom. rev., Flexibacter psychrophilussp. nov., nom. rev., and Flexibacter maritimus Wakabayashi, Hikida, and Masumura 1986. International journal of systematic and evolutionary microbiology.39(3): 346-354.

    Buller N.B. (2004). Bacteria from fish and other aquatic animals: a practical identification manual. Cabi.

    Chang L., Pate J.L. & Betzig R. (1984). Isolation and characterization of nonspreading mutants of the gliding bacterium Cytophaga johnsonae. Journal of bacteriology. 159: 26-35.

    CLSI (2016). Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. 3rd ed. CLSI guideline M45. Wayne, PA: Clinical and Laboratory Standards Institute.

    CLSI. (2020). Performance Standards for Antimicrobial Testing of Bacteria Isolated from Aquatic Animals. 3rded. CLSI supplement VET04. Wayne, PA: Clinical and Laboratory Standards Institute.

    Declercq A.M., Haesebrouck F., Van den Broeck W., Bossier P. & Decostere A. (2013a). Columnaris disease in fish: a review with emphasis on bacterium-host interactions. Veterinary research. 44: 1-17.

    Declercq A., Boyen F., Van Den Broeck W., Bossier P., Karsi A., Haesebrouck F. & Decostere A. (2013b). Antimicrobial susceptibility pattern of Flavobacterium columnareisolates collected worldwide from 17 fish species. Journal of fish diseases. 36: 45-55.

    Dong H., LaFrentz B., Pirarat N. & Rodkhum C. (2015). Phenotypic characterization and genetic diversity of Flavobacterium columnareisolated from red tilapia, Oreochromis sp., in Thailand. Journal of fish diseases. 38: 901-913.

    Dong H., Senapin S., LaFrentz B. & Rodkhum C. (2016). Virulence assay of rhizoid and non‐rhizoid morphotypes of Flavobacterium columnarein red tilapia, Oreochromissp., fry. Journal of fish diseases. 39: 649-655.

    FAO (2020). The State of World Fisheries and Aquaculture: Sustainability in action.Rome,Itali. p. 2020. https://doi.org/10.4060/ca9229en

    Koppang E.O., Kvellestad A. & Fischer U. (2015). Fish mucosal immunity: gill Mucosal health in aquaculture. Elsevier.pp. 93-133.

    Kunttu H., Jokinen E., Valtonen E. & Sundberg L.R. (2011). Virulent and nonvirulent Flavobacterium columnarecolony morphologies: characterization of chondroitin AC lyase activity and adhesion to polystyrene. Journal of Applied Microbiology. 111: 1319-1326.

    Kuo S.C., Chung H.Y. & Kou G.H. (1981). Studies on artificial infection of the gliding bacteria in cultured fishes. Fish Pathology. 15: 309-314.

    Lu Z., Gao R., Duan Y., Han R., Guo W., Dan X. & Li Y. (2021). Isolation and genetic characterization of Flavobacterium columnarefrom grass carp, Ctenopharyngodon idellus, in China. Aquaculture. 541: 736762.

    Shen Y., Wang L., Fu J., Xu X., Yue G.H. & Li J. (2019). Population structure, demographic history and local adaptation of the grass carp. BMC genomics.20(1): 1-16.

    Shi F., Lu Z., Yang M., Li F., Zhan F., Zhao L., Li Y., Li Q., Li J. & Li J. (2021). Astragalus polysaccharides mediate the immune response and intestinal microbiota in grass carp (Ctenopharyngodon idellus). Aquaculture. 534: 736205.

    Tohmee N. & Deemagarn T. (2013). Flavobacterium columnareisolated from brains of pond culture Nile tilapia in Thailand. 38th ICVS Conference, Bankok, Thailand.

    Từ Thanh Dung, Nguyễn Anh Tuấn & Nguyễn Thị Tiên (2012). Nghiên cứu tác nhân gây bệnh trắng đuôi trên cá tra (Pangasianodon hypophthalmus) và giải pháp điều trị. Tạp chí Khoa học Trường Đại học Cần Thơ. 22c: 136-145.

    Welker T.L., Shoemaker C.A., Arias C.R. & Klesius P.H. (2005). Transmission and detection of Flavobacterium columnarein channel catfish Ictalurus punctatus. Diseases of aquatic organisms. 63:129-138.