Received: 25-09-2023 / Accepted: 05-01-2024
The purpose of this investigation was to select biofilm-forming bacteria which can degrade or transform crude oil components leading to the solution of oil contamination causing by exploitation, transportation and use of oil and oil products. To gain this aim, several approaches such as enrichment to isolate and identify the bacteria that can utilize oil components and form biofilm and to assess oil degradation capacity of biofilm type in comparison with planktonic type of the strains. As results, the biofilm–forming Rhizobium sp. DG2 was isolated from oil contaminated sediment samples taken in petroleum storage in Ducgiang, Hanoi. The biofilm of this strain was capable of degrading 44.8; 76.0, 62.0, 73.0 and 75.0 % of diesel oil, anthracene, naphthalene, phenanthrene and pyrene, respectively, with the initial concentrations of 4.786 g/L for diesel oil and 200 ppm for the others. In contrast, the plantonik Rhizobium sp. DG2 in free form degraded only 35.4; 65.1; 54.5; 54.6 and 64.2 % of these components. The obtained results suggest the potential of using biofilm-forming bacteria in general and Rhizobium sp. in particular to treat oil pollution.