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• Molecular Marker for Using to Detect Fragrant Gene in Aromatic Rice Breeding

  Duong Xuan Tu, Nguyen Van Khoi, Le Thi Thanh, Nguyen Thi Huong, Nguyen The Duong, Tran Thi Dieu, Phan Huu Ton

  Received: 29-04-2014 / Accepted: 18-07-2014

   Abstract PDF (VIETNAMESE)

  DNA markers RG28, RM223, RM342, L06 and BADH2 comprising 4 primers EAP, ESP, IFAP, INSP have been reported closely linked to fgr gene located on chromosome 8 controlling 2AP synthesis, major materials producing aroma in rice. In our report, marker BADH2 (4 primers EAP, ESP, IFAP, INSP) yielded in high polymorphism and could accurately differentiate between fragrant and none-fragrant rice material. Analysis of F2 population derived from cross between aromatic and non-aromatic rice varieties using this marker showed approximate ratio of segregation of 1: 2 : 1 . Using this marker, from 92% to 95% of F2 individuals expressing aroma in grains were identified as homozygotes for fgr gene. These results confirmed the accuracy of BADH2 marker with 4 primers EAP, ESP, IFAP, INSP to detect fragrant gene in aromatic rice breeding materials.

• Selection of Thinner Pericarp Thickness for Quality of Fresh Waxy Corn

  Tran Thi Thanh Ha, Nguyen Thi Hong Ngat, Nguyen Van Ha, Duong Thi Loan, Vu Thi Bich Hanh, Vu Van Liet

  Received: 19-02-2013 / Accepted: 22-04-2013

   Abstract PDF (VIETNAMESE)

  Waxy corn is commonly eaten fresh in Asian countries including Vietnam. Selection for thinner pericarp is a priority for enhancing tenderness in fresh waxy corn breeding. We have evaluated 48 of local maize cultivars and inbred lines in order to identify genetic materials that have thinner perricap for the waxy maize breeding with improved quality. The 48 cultivars and inbred lines were evaluated in the field experiment with two replication in 20012 Autumn-Winter season. Kernel pericarp thickness of cultivars and inbed lines (measured by micrometer) ranged between 51,6 to 118,9 µm. Six potential lines and cultivars with desirable pericarp thinness are D27, D34, D36, D14, D22 and D35 (D27 with 51.6 µm). Using SSR markers 28 cultivars and inbred lines were detected to have QTL controlling thinner pericarp traits. Based on phenotypic evaluatione and genetic markers, six cultivars and inbred lines, D14, D22, D27, D47, D36 and D44, possessing desirable agronomic characteristics, thinner pericarp were selected for high quality waxy maize breeding programme in Vietnam.