Received: 30-07-2014 / Accepted: 13-03-2015
In order to produce a Dendrobiumlinesresistant toCyMV (Cymbidium mosaic virus), protocorm like bodies (PLBs) of DendrobiumSoniawere infected with Agrobacterium tumefaciensC58 carrying plant transgenic vector pK7GWIWG2 (II)/CP, containing RNAi structure of coat protein gene (CP) alone or in combination with ORF2-4 gene of CyMV. The transformed PLBs were disinfected with cefotaxime 300mg/l and screened for kanamycin 500mg/l. Viable PLB-derivedlines were obtained after the screening. The presence of the target gene in these lines was confirmed by PCR.