Semen Quality and Bacterial Growth in Boar Semen Extender Preserved at Low Temperature

Received: 19-02-2021

Accepted: 13-07-2021

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Issue: Vol. 19 No. 9 (2021)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Doanh, B., Yen, D., Hai, D., & Dang, P. (2024). Semen Quality and Bacterial Growth in Boar Semen Extender Preserved at Low Temperature. Vietnam Journal of Agricultural Sciences, 19(9), 1150–1158. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/875

Semen Quality and Bacterial Growth in Boar Semen Extender Preserved at Low Temperature

Bui Huy Doanh (*) 1 , Dinh Thi Yen 1 , Dang Thai Hai 1 , Pham Kim Dang 1

  • 1 Khoa Chăn nuôi, Học viện Nông nghiệp Việt Nam

    • Keywords

    Semen, preservation, semen quality, temperature, bacteria

    Abstract


    This study was to evaluate the effect of hypothermic preservation strategy on semen quality as well as the bacterial growth in antibiotic-free extender at 5°C. Diluted boar semen containing antibiotic were stored in BTS and AndroStar Premium extenders at 17°C while antibiotic-free semen were stored at 5°C. Sperm motility, proportion of abnormal spermatozoa and bacterial growth after 24, 72 and 120 hours preservation were investigated. The results showed that the boar spermatozoa preserved in antibiotic-free AndroStar Premium extender at 5°C maintained high motility above 75% after 5 days of preservation and did not significantly differ from the control stored at 17°C in extender containing antibiotic (P <0.05). The proportion of abnormal spermatozoa in semen samples stored in AndPre extender at 5°C was similar to the samples stored in BTS and AndPre extenders at 17°C (P >0.05). The number of aerobic bacteria in semen samples stored at 5°C in antibiotic-free extenders was lower than 103CFU/ml after 24, 72 and 120 hours preservation and did not differ significantly with samples containing antibiotic extender stored at 17°C (P >0.05). Therefore, semen preservation in antibiotic-free AndPre extender at low temperatures might be applied to minimize the use of antibiotics in livestock.

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