Received: 26-08-2020
Accepted: 18-01-2020
DOI:
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Detection of anti-Toxocara spp. and Ascaris suum Antibodies in Naturally Infected Free-range Chickens
,
Nguyen Thi Hop
3
,
Do Trung Dung
3
,
Pham Thi Toi
4
,
Dong The Anh
2
,
Nguyen Van Phuong
2
,
Nguyen Thi Hong Chien
2
Keywords
Ascarissuum, ELISA, free-rangechicken, Toxocaraspp
Abstract
The purpose of this study was to detect anti-Toxocara spp. and Ascaris suum antibodies in freely raised chickens. Two hundred and fifty-one serum samples were collected at the fresh market (Trau Quy, Gia Lam, Ha Noi) and some small-scale free-range chicken farms (Bac Giang Province). Indirect ELISA was applied using As-SWAP crude antigen to screen and choose serum samples which showed positive reactions with anti-ascarid antibodies and using Tci-ES and Asm-ES antigens to discriminate infecting species. Next, Toxocara Western Blotting Kit was applied to confirm Toxocara infection, and pre-adsorbed ELISA was conducted to confirm A. suum infection. The results showed that: 38.65% of serum samples contained anti-ascarid antibodies. Additionally, the result of discrimination of infecting species assumed that 11.55% and 2.79% samples containing anti-Toxcocara spp. and anti-A. suum antibodies, respectively. This is the first study to detect Toxocara spp. and A. suum in naturally infected free-range chickens. The attained results of this study provides important information for chicken farmers in protecting themselves in order to avoid exposure to ascarid egg-contaminated soil. Besides, it gives evidence of the risk of getting A. suum infection in humans in Vietnam.
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