Improvement of the Efficiency of Bovine in vitroFertilization Embryo Production

Received: 18-08-2020

Accepted: 07-12-2020

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Issue: Vol. 19 No. 1 (2021)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Lanh, D., Anh, N., Chi, H., Nhung, N., Truong, N., Long, S., & Thanh, N. (2024). Improvement of the Efficiency of Bovine in vitroFertilization Embryo Production. Vietnam Journal of Agricultural Sciences, 19(1), 25–32. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/762

Improvement of the Efficiency of Bovine in vitroFertilization Embryo Production

Do Thi Kim Lanh (*) 1 , Nguyen Thi Ngoc Anh 1 , Hoang Thi Kim Chi 1 , Nguyen Thi Hong Nhung 1 , Nguyen Ba Truong 1 , Su Thanh Long 1 , Nguyen Van Thanh 2

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Phòng Thí nghiệm trọng điểm Công nghệ tế bào, Viện Chăn nuôi

    • Keywords

    Bovine oocyte, in vitrofertilization, cumulus cell, bovine embryo

    Abstract


    The present study was conducted to improve the efficiency of in vitroproduction of bovine embryos. Bovine oocytes were matured either in BO-IVM or TCM199 medium then evaluated the maturation rates by orcein staining. After maturation, bovine oocytes were in vitro fertilized (IVF) in a medium with frozen-thawed sperms at different concentrations (1, 2, or 5 ×106sperms/ml) for 12 hours. The fertilization process was then evaluated at 3, 6 or 12h of oocytes and sperms co-incubation to determine the optimal sperm concentration and co-incubation time. Denuded zygotes were then transferred into SOF medium with or without pre-cultured cumulus cell to evaluate the cleavage rates at day 3 and blastocyst formation rates at day 7. There was no significant difference in the maturation rate of bovine oocytes cultured in BO-IVM or TCM199. Fertilization of bovine oocytes with frozen-thawed sperms at 2 ×106sperms/ml for 6 h significantly improve the fertilization rate and blastocyst formation rate (69.87% and 29.1%). The cleavage rate and blastocyst rate of cattle fertilized oocytes cultured in SOF medium with pre-cultured cumulus cells were higher than those of embryos cultured without cumulus cells (76.34% and 34.16% vs 54.23% and 27,22%, P <0.05). The optimal conditions for in vitro produced cattle embryos can be performed as the maturation of the oocytes with TCM199 medium, fertilization at 2 ×106sperms/ml for 6 hours, then cultured in SOF medium with pre-cultured cumulus cells.

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