Study on Improving the Synthesis of Multi-enzymes (Cellulase, α-Amylaseand Glucoamylase) from Aspergillus nigerA45.1 by Mutation and Optimal Condition of Solid State Fermentation

Received: 17-07-2019

Accepted: 14-11-2019

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Issue: Vol. 17 No. 8 (2019)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Huong, D., Dang, P., & Hanh, V. (2024). Study on Improving the Synthesis of Multi-enzymes (Cellulase, α-Amylaseand Glucoamylase) from Aspergillus nigerA45.1 by Mutation and Optimal Condition of Solid State Fermentation. Vietnam Journal of Agricultural Sciences, 17(8), 666–678. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/591

Study on Improving the Synthesis of Multi-enzymes (Cellulase, α-Amylaseand Glucoamylase) from Aspergillus nigerA45.1 by Mutation and Optimal Condition of Solid State Fermentation

Duong Thu Huong (*) 1 , Pham Kim Dang 1 , Vu Van Hanh 2

  • 1 Khoa Chăn nuôi, Học viện Nông nghiệp Việt Nam
  • 2 Viện Công nghệ sinh học, Viện Hàn lâm Khoa học Việt Nam

    • Keywords

    Mutant, enzyme, solid state ferrmentation

    Abstract


    The study was conducted to enhance fungi strain and optimize the condition of solid state fermentation for improving the synthesis of multi-enzymes (cellulase, α-amylase and glucoamylase). Aspergillus nigerA45.1 strain was selected for simultaneous mutation treatment by UV and N-methyl-N -nitro-N-nitrosoguanidine (NTG) with mutagenic doses of 0, 30, 60, 90, 120, 150 and 180 minutes to enhancce the secretion of multil-enzymes. After mutation treatments, the Aspergillus sp. GA15 strain with the highest activity of glucoamylase, alpha amylase and celulase enzymes was optimized the fermentation condition to produced multi-enzyme by solid state fermentation. The result found the optimal condition to ferment Aspergillus sp. GA15 was obtained in 5 days fermentation of 2 days old fungi with wheat bran substrate, 1% glucose, 1% urea supplymentation, 50% moisture, pH 5.5 and 30C. Particularly, the activity of glucoamylase, alpha amylase and celulase enzyme was 76,75 U/g; 50 U/g and 40,11 U/g, respectively, which was higher 2,8; 1,29 and 3,3 times compared to normal conditions.

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