Received: 26-07-2018
Accepted: 03-12-2018
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In vitropropagation of Taiwanese Sweet Mulberry
Keywords
Sweet mulberry, in vitropropagation, Buffalo head 502, BA, IBA
Abstract
In vitropropagation of Taiwanese sweet mulberry was carried out to determine technical parametersinthe production of high quality planting materialsfor expanding cultivated area of the newly introducedfruit tree. One-month-old shoots of two-year-old Taiwanese sweet mulberry plantswere used as initial materialsfor in vitroculture. Results showed that BA at the concentration of 3mg/l in MS medium was suitable for stimulating in vitroshoots from stem nodes with highrate(83.33%). BA at 3 mg/l in combination with0.5 mg/l IBA and 10% coconut water gave the best shoot multiplication rate (2.93 shoots/explant) and the highest shoot quality after 4 weeks of culture. The highest rooting percentage at 96.67% of shoots was observed after 4 weeks onMS medium containing 0.5 mg/l IBA. The plantlets were successfully acclimated in sandy substrate with a survival rate of 80%. The liquid fertilizer “Buffalo Head 502” foliar spary 1 time/weekyielded good quality plantlets suitable for transplanting into pots.This in vitropropagation process is highly effective and easy to apply at production scale.
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