Preliminary Evaluation of Potential Use of Simple Sequence Repeats (SSRs) in Studying Genetic Relationships among Isolates of Aspergillus spp.

Received: 01-10-2012

Accepted: 12-12-2012

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Issue: Vol. 10 No. 7 (2012)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Hai, N., Quynh, D., Thanh, B., Thuong, N., Hoa, N., Chinh, N., & Nghiem, D. (2024). Preliminary Evaluation of Potential Use of Simple Sequence Repeats (SSRs) in Studying Genetic Relationships among Isolates of Aspergillus spp. Vietnam Journal of Agricultural Sciences, 10(7), 1032–1043. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/26

Preliminary Evaluation of Potential Use of Simple Sequence Repeats (SSRs) in Studying Genetic Relationships among Isolates of Aspergillus spp.

Nguyen Khac Hai (*) 1 , Do Hai Quynh 1 , Bui Thi Thanh 1 , Nguyen Van Thuong 1 , Nguyen Ngoc Hoa 1 , Nguyen Ngoc Chinh 1 , Dang Xuan Nghiem 2

  • 1 Khoa Công nghệ Sinh học, Trường Đại học Nông nghiệp Hà Nội
  • 2 Khoa Công nghệ Sinh học, Đại học Nông nghiệp Hà Nội

    • Keywords

    Aspergillus oryzae, Aspergillus spp., molecular markers, moulds, SSRs

    Abstract


    This study is aimed at exploring simple sequence repeats (SSRs) arrays and their polymorphism in Aspergillus oryzae, laying the foundations for the application of those sequences as molecular markers in investigation of genetic relationships among Aspergillus spp. isolates and in identification of a certain Aspergillus spp. strain. In silico data reveals that there is a total of 841 SSRs in the nuclear genome of Aspergillus oryzae. The density of those SSRs on 8 chromosomes ranges from 16 to 26 SSRs per Mb. The perfect SSRs sequences with 100% conserved repeats are in the majority, accounting for 44,6% of all 841 with the conservation bigger than 70%. The successful amplification of 17 SSR and iSSR loci revealed a high average number of alleles of 4,88 and a high genetic diversity with the average PIC value of 0,6 for each locus. Especially, there are specific patterns of allele number and length for each groups of the investigated moulds. This results confỉmed a high potential application of those SSR loci for detecting the presence of those moulds in human food and animal feed.

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