Isolation and Screening of Wood Decaying Fungi Producing Laccase

Received: 28-01-2015

Accepted: 09-10-2015

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Issue: Vol. 13 No. 7 (2015)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Thuy, T., Giang, N., Bang, N., & Trang, P. (2024). Isolation and Screening of Wood Decaying Fungi Producing Laccase. Vietnam Journal of Agricultural Sciences, 13(7), 1173–1178. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1552

Isolation and Screening of Wood Decaying Fungi Producing Laccase

Trinh Thi Thu Thuy (*) 1 , Nguyen Van Giang 2 , Nguyen Ngoc Bang 3 , Pham Thu Trang 2

  • 1 Khoa Công nghệ Sinh học, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 3 Khoa Chăn nuôi và Nuôi trồng thủy sản, Học viện Nông nghiệp Việt Nam

    • Keywords

    Enzyme activity, wood-decaying fungi, laccase

    Abstract


    Laccase enzyme is a multicopper enzyme of the lignolytic enzyme system which has capability to oxidize diphenol and related compounds. It is widely used in processing of agricultural by-products andinwater treatment. This study was conducted to isolateand screen fungal strains which have laccase activity from the natural decaying wood samples. Samples of decayed woods were collected from different locationsandthe fungi were isolated, cultured and purified. Test for presence of laccase and enzyme activity were used to screen laccase-producing fungi strains withthe high laccase activity. Five fungal strains, designated as BN1, BN2-1, BN2-2, DA3-1 and BV1 were isolated, cultured and morphologically described(colonies, mycelium and spores). These strains showed highlaccase activity withBV1 expressinghighest activity(247.20 U/ml) and highest rate of enzyme activity per dry weight after 5 days of culture (E/m, 54.04 U/mg). BV1 strainwas tentatively classified as a species of the genus Meruliporia.

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