Received: 25-07-2014
Accepted: 09-10-2015
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Calcineurin B-Like Protein Cbl4 and Cbl5 Participate in SignalTransductioninGerminating Rice (Oryza sativaL.) under Water Submergence
,
Pierdomenico Perata
2
,
Chiara Pucciariello
2
Keywords
OsCBL(calcineurin B-likein rice), OsCIPK15(calcineurin B-like interacting protein kinase 15), Submergence, Oryza sativa
Abstract
OsCIPK15is a key regulator of starch degradation in rice due to submergence. This gene receives submergence signal via calcineurin B-like (OsCBL) proteins, which act as the Ca2+sensors, and whose role has yet to be established. In the present study, we compared the transcriptional regulation of CBLsin germinating rice embryos under water submergence. The trans-activation of OsCBLpromoters was tested by examining the effects of Ca2+. We used rice protoplasts to study the OsCBLs-OsCIPK15 protein interactions. We found that OsCBL4and OsCBL5were transcriptionally regulated under submergence.The Ca2+sensors OsCBL4 and OsCBL5 wereinvolved in this system and interactedon the plasma-membrane with OsCIPK15 in Ca2+dependent manner. Our results suggest that Ca2+signaling formed under submergence are inducedby OsCBL4and OsCBL5 which then interact with OsCIPK15 to activate genes involving instarch catabolism to provide energy for rice germination.
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