Received: 17-01-2016
Accepted: 15-07-2016
DOI:
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in vitroPropagation of Coelogyne mooreana Sander Ex Rolfe
,
Dang Thi Tham
1
,
Nguyen Thi Thanh Hang
1
,
Dinh Van Khiem
1
,
Nong Van Duy
1
,
Vu Kim Cong
1
,
Quach Van Hoi
1
,
Tran Thai Vinh
1
Keywords
Coelogyne mooreanaSander ex Rolfe, in vitromicropropagation, shoot tips, protocorm
Abstract
In this study, in vitromass propagationof Coelogyne mooreanaSander ex Rolfe, an endemic orchid (of Vietnam) with its beautiful large,white flowers and fragrance having horticultural potential was accomplished through protocorm formation from shoot tips and subsequent plant regeneration. In the first procedure, shoot tips from in vitrogerminated seedlings were cultured on Murashige and Skoog medium (MS) supplemented with arange of 6- benzylaminopurine (BA) in combination with naphthalene acetic acid (NAA) to determine the best medium for the protocormformation. The results showed that MS medium supplemented with 1.0 mg/l BA and 0.5 mg/l NAA was the most suitable for protocorm formation (6.62 protocorms và formation rate of 86.63%). For subculture, maximum growth of shoots (14 shoots, 2.72 cm height and 3 leaves/shoot) was obtained on MS medium supplemented with0.3 mg/l NAA and 2.0 mg/l BA. The highest frequency of rooting (100%) was observed on ½MS medium supplemented with 20 g/l sucrose, 8 g/l agar, 1 g/l activated charcoal (AC),10% coconut water (CW) and 0.5 mg/l NAA. On this medium, the seedlings reached an average length of 1.66 cm with 3.8 roots per seedling. A successful protocol for micropropagation by shoot tips will contribute to the development of a suitable management program of C. mooreanaSander ex Rolfe.
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