DevelopingDirect Dry LAMP Method forField Diagnosis of African Swine Fever

Received: 06-11-2023

Accepted: 07-03-2024

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Issue: Vol. 22 No. 3 (2024)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Ngan, M., Viliddeth, S., Giang, T., Hieu, D., Giang, N., & Anh, D. (2024). DevelopingDirect Dry LAMP Method forField Diagnosis of African Swine Fever. Vietnam Journal of Agricultural Sciences, 22(3), 322–330. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1284

DevelopingDirect Dry LAMP Method forField Diagnosis of African Swine Fever

Mai Thi Ngan (*) 1 , Souriya Viliddeth 1 , Tran Thi Huong Giang 1 , Dong Van Hieu 1 , Nguyen Thi Huong Giang 2 , Dang Huu Anh 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Chăn nuôi Thú y, Trường Đại học Nông Lâm Bắc Giang

    • Keywords

    African Swine Fever, one-step dry LAMP, two-step dry LAMP, storage conditions

    Abstract


    African swine fever (ASF) is caused by the ASF virus (ASFV) of the family Asfarviridae with a mortality rate of up to 100%. Currently, studies on the LAMP method (Loop-mediated isothermal amplification) for ASF diagnosis are based onwet LAMP, which is not feasible for field application due to the requirement of cold conditions. This study aimedto evaluate the effectiveness of dry LAMP methods to support early field diagnosis of ASF. The gold standard real-time PCR method was used to evaluate the sensitivity and specificity of the direct dry LAMP method. The results show that the two-step dry LAMP method wasmore effective in DNA amplification than the one-step dry LAMP method. Both dry LAMP methods showed good DNA amplification efficiency in both storage conditions after 2 months.The sensitivity and specificity of the two-step dry LAMP method after 2.5 months at 4C were 88.1% and 100%. Thus, the dry LAMP method is promising as an early diagnosis tool for ASF, helping to support the screening of infected individuals at the field and to improve the effectiveness of prevention and control of ASF.

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