Improvement the Yield and Quality of Lingzhi (Ganodermaspp.) by Mutation using Gamma Ray (Cobalt 60)

Received: 14-09-2023

Accepted: 25-12-2023

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Issue: Vol. 21 No. 12 (2023)

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NÔNG HỌC

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Giang, N., Hoi, P., Hai, N., & Hang, N. (2024). Improvement the Yield and Quality of Lingzhi (Ganodermaspp.) by Mutation using Gamma Ray (Cobalt 60). Vietnam Journal of Agricultural Sciences, 21(12), 1601–1611. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1231

Improvement the Yield and Quality of Lingzhi (Ganodermaspp.) by Mutation using Gamma Ray (Cobalt 60)

Nguyen Thi Giang (*) 1 , Pham Xuan Hoi 1 , Nguyen Thi Hong Hai 1 , Nguyen Thi Hang 1

  • 1 Viện Di truyền Nông nghiệp

    • Keywords

    Lingzhi (Ganoderma lucidum), irradiation, gamma ray, polysacharide, triterpenoid

    Abstract


    In this work, in order to improve the yield and quality of Ganoderma lingzhis in Vietnam, three lingzhi varieties, DT, D18 and D20, were irradiated with gamma rays (60Co) on the mycelium at different doses (0.25, 0.5, 0.75, 1.0; 1.25, 1.5and 2kGy). The evaluation results selected five mutant strains DTL0,5; DTL0,75; DTL1; DTL1,25 and D18L0,75, these strains showed strong mycelium, early fruiting body formation, and early harvest maturity and dry mushroom yield increased by 13-16% compared to the control. The polysaccharide content of DTL0.75; DTL1; DTL1.25 and thetriterpenoid content of DTL1.25 and D18L0.75 were considerably higher than the parental strains. The results indicated that the irradiation intensity had significantly effect on the development as well as the active pharmaceutical ingredients of the mutant strains, specifically, the irradiation doses from 0.5-1.25kGray had a positive effect growth, productivity and quality of mutant strains; and irradiation dose ≥ 1.5kGray is not beneficial for the mycelial development of mutant strains.

    References

    Anitha R. (1998). Strain improvement in Oystermushroom (Pleurotusspp.). MSc (Ag) thesis, Kerala Agricultural University, Thrissur. 92p.

    Boh B., Berovic M., Zhang J. & Zhi-Bin L. (2007). Ganoderma lucidum and its pharmaceutically active compounds. National Library of Medicine. 13: 265-301. doi: 10.1016/S1387-2656(07)13010-6.

    Chen Yi, Ming-Yong Xie & Xiao-Feng Gong (2007). Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. Journal of Food Engineering. 81(1): 162-170.

    Dong Y., Miao R., Feng R., Wang T., Yan J., Zhao X., Han X., Gan Y., Lin J., Li Y., Gan B. & Zhao J. (2022). Edible and medicinal fungi breeding techniques, a review: Current status and future prospects. Current Research in Food Science.

    El Sheikha A.F. (2022). Nutritional profile and health benefits of Ganoderma lucidum “Lingzhi, Reishi, or Mannentake” as functional foods: Current scenario and future perspectives. Foods. 11(7): 1030.

    El-Ramady H., Abdalla N., Badgar K., Llanaj X., Törős G., Hajdú P., Eid Y. & Prokisch J. (2022). Edible mushrooms for sustainable and healthy human food: Nutritional and medicinal attributes. Sustainability. 14(9): 4941.

    Esser K. (1971). Application and importance of fungal genetics for industrial research. In: Proceedings of the symposium on use of radiation and radioisotopes for industrial microorganisms, 29 March - 1 April, Vienna, Austria. pp. 83-91.

    IAEA (International Atomic Energy Agency) (1992). Irradiation of spices herbs and other vegetable seasonings. A compilation of technical data for its authorization and control. IAEA-TECDOC. 639: 16-17.

    IAEA (International Atomic Energy Agency) (2009). Developments in mutation assisted plant breeding. Retrieved from ttp://www.iaea.org/About/Policy/ GC/GC53/GC53InfDocuments/English/gc53inf-3-att1_en.pdf. on Aug 18, 2023.

    Liu L., Van-Zanten L., Shu Q.Y. & Maluszynski M. (2004). Officially released mutant varieties in China. Mutation Breeding Review (IAEA Newsletter). 14: 1-64.

    Liu S.R., Ke B.R., Zhang W.R., Liu X.R. & Wu X.P. (2017). Breeding of new Ganoderma lucidum strains simultaneously rich in polysaccharides and triterpenes by mating basidiospore-derived monokaryons of two commercial cultivars. Scientia Horticulturae. 216: 58-65.

    Ma, Y., Zhang, Q., Zhang, Q., He, H., Chen, Z., Zhao Y., Wei D., Kong M. & Huang Q. (2018). Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy. PLoS One. 13(9): e0204266

    Nakagawa H. (2009). Induced mutations in plant breeding and biological researches in Japan. Induced Plant Mutations in the Genomics Era. pp. 51-58. Retrieved from http://www.fnca.mext.go.jp/ english/ mb/docdb/ data/jpn035.pdf on Aug 18, 2023.

    Nguyen B.T.T., Ngo N.X., Van Le V., Nguyen L.T., Kana R. & Nguyen H.D. (2019). Optimal culture conditions for mycelial growth and fruiting body formation of Ling Zhi mushroom Ganoderma lucidum strain GA3. Vietnam Journal of Science, Technology and Engineering. 61(1): 62-67.

    Peng R., Fu Y.Z., Zou J., Qiu H., Gan L.T., Yi H.L. & Luo X.Y. (2016). Improvement of polysaccharide and triterpenoid production of Ganoderma lucidumthrough mutagenesis of protoplasts. Biotechnol. Biotechnol. Equip. 30(2): 381-387. doi.org/10.1080/13102818.2015.1133254.

    Zheng S., Zhang W. & Liu S. (2020). Optimization of ultrasonic-assisted extraction of polysaccharides and triterpenoids from the medicinal mushroom Ganoderma lucidum and evaluation of their in vitroantioxidant capacities. PLoS One. 15(12): e0244749.