Genetic diversity analysis of 30 accessions of lotus (Nelumbo spp.) by RADP and ISSR markers

Received: 09-12-2022

Accepted: 22-05-2023

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Issue: Vol. 21 No. 5 (2023)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

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Tuoi, N., Ngoc, P., Hong, N., Loi, H., Hue, N., Cuong, P., & Son, D. (2024). Genetic diversity analysis of 30 accessions of lotus (Nelumbo spp.) by RADP and ISSR markers. Vietnam Journal of Agricultural Sciences, 21(5), 605–616. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1128

Genetic diversity analysis of 30 accessions of lotus (Nelumbo spp.) by RADP and ISSR markers

Ngo Thi Hong Tuoi (*) 1 , Pham Thi Ngoc 1 , Nguyen Thi Bich Hong 1 , Ha Thi Loi 2 , Nguyen Thi Hue 2 , Pham Van Cuong 3 , Dinh Truong Son 4, 2

  • 1 Khoa Nông học, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 3 Phòng Đào tạo - Quản lý Khoa học, Trường Đại học Hoa Lư
  • 4 Viện Sinh học Nông nghiệp, Học viện Nông nghiệp Việt Nam

    • Keywords

    Lotus, Nelumbo spp., ISSR and RAPD markers, genetic diversity

    Abstract


    Lotus (Nelumbo spp., Nelumbonaceace family) is an important horticultural plant, with its uses ranging from ornamental, nutritional to medicinal values, and has been widely used in Vietnam. This study aimed to evaluate the genetic diversity of 30 lotus accessions collected in some provinces in Vietnam by using RAPD and ISSR markers. 12 RAPD and 18 ISSR successfully detected 178 loci, among these, 115 were polymorphic loci and 4084 clear and scorable bands were obtained. The similarity coefficient of 30 accessions ranged from 0.65 to 0.93 when combined both RAPD and ISSR markers. At the genetic similarity of 0.80, 30 lotus accessions could be divided into 4 distinct clusters. These results showed that the genetic diversity of 30 collected lotus accessions were very high. The 30 collected accessions are valuable source for the conservation and development of lotus genetic resources as well as for the breeding of new lotus varieties.

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