Identification of SSR Markers Linked to sh2and su1Genes in Sweetcorn Lines

Received: 09-08-2022

Accepted: 02-03-2023

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Issue: Vol. 21 No. 2 (2023)

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NÔNG HỌC

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Duc, N., Trung, N., Tuan, P., Anh, N., Hang, P., Hoi, P., & Liet, V. (2024). Identification of SSR Markers Linked to sh2and su1Genes in Sweetcorn Lines. Vietnam Journal of Agricultural Sciences, 21(2), 149–160. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1110

Identification of SSR Markers Linked to sh2and su1Genes in Sweetcorn Lines

Nguyen Trung Duc (*) 1 , Nguyen Quoc Trung 2 , Pham Quang Tuan 1 , Nguyen Thi Nguyet Anh 1 , Pham Thu Hang 3 , Pham Xuan Hoi 3 , Vu Van Liet 4

  • 1 Viện Nghiên cứu và Phát triển cây trồng, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 3 Viện Di truyền Nông nghiệp, Viện Khoa học Nông nghiệp Việt Nam
  • 4 Khoa Nông học, Học viện Nông nghiệp Việt Nam

    • Keywords

    SSR markers, sweet corn, su1, sh2, sh2sh2su1su1

    Abstract


    The objectives of this study were to identify SSRmarkers closely associated with shrunken2 (sh2)andsugary1 (su1) genesandapplication forselecting target genotypes on 30 S3 sweet corn lines. Four SSR markers closely associated with the sh2gene (umc1320, umc2276, umc1273, bnlg1257) and four SSR markers closely associated with the su1gene (umc1142, umc1031, bnlg1937, umc2061) were investigated in three check lines. The results showed that the markers umc2276 and umc1031 were suitable to identify the sh2and su1genes, respectively. Using these markers for screeningon 30 S3 sweet corn lines identified 7 lines homozygous for recessive gene sh2sh2(D04, D09, D13, D21, D22, D24, D25), 2 lines homozygous for recessive gene su1su1(D19, D30) and 2 lines double homozygous recessive for sh2sh2su1su1(D11, D14). Phenotypic evaluation confirmed the similarity to genotypes and proved high accuracy of the selected molecular markers. Total soluble solids of the sh2sh2su1su1lines were higher than sh2sh2lines and superior to that of the su1su1lines. These SSR markers and 11 selected genotypes played a vital role for the rapid development of high-quality inbred lines for specialty corn hybrid breeding in Vietnam.

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