Received: 01-02-2023
Accepted: 07-03-2023
DOI:
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An Initial Study on oil Pollution Removal Product using Biofilm Forming Bacteria Attached on Husk Biochar
,
Do Thi Lien
2
,
Cung Thi Ngoc Mai
2
,
Tran Thi Dao
3
,
Tran Phuong Minh
4
,
Le Thi Nhi Cong
1
Keywords
Biochar, biofilm, biodegradation, fermentation, oil polluted waste water
Abstract
Oil contaminants create remarkably environmental pollution problems because they have high toxicity and are recalcitrant to the environment.Bioremediation is an attractive alternative of utilizing bacteria to remove oil contaminants. In the present study, bacterial strains producing biofilm capable of degrading oil contaminants/ components were screened and some fermentation conditions with rice husk biochar as carrierto produce biodegradation product to remove oil contaminants were tested. Several microbial traditional methods such as screening, biofilm formation, fermentation, microbe denstity and total oil removal by TCVN 4582-88 were conducted. As the results, four biofilm forming bacterial strains highly capable of degrading and metabolizing hydrocarbon compounds were selected, i.e. Acinetobacter baumanniiQN01, Rhizobium sp. DG2, Rhodococcussp. BN5 and Stenotophomonas maltophilia QNG02. Fermentation on husk biochar to produce oil degrader product showed that suitable fermentation temperature was 40C and the product moisture was 40%. The product exhibited high removal efficiency (99%) for diesel oil contaminants after 7 day-incubation at the initial concentration of 10g/kg oil polluted soil.
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