Isolation and Characterization of Sclerotium rolfsiiStrains Causing Stem Rot Disease in Peanut

Received: 04-08-2022

Accepted: 20-12-2022

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Issue: Vol. 20 No. 12 (2022)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Truong, N., Dao, T., Trang, T., Huyen, N., Anh, N., & Canh, N. (2024). Isolation and Characterization of Sclerotium rolfsiiStrains Causing Stem Rot Disease in Peanut. Vietnam Journal of Agricultural Sciences, 20(12), 1659–1671. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1082

Isolation and Characterization of Sclerotium rolfsiiStrains Causing Stem Rot Disease in Peanut

Nguyen Xuan Truong (*) 1 , Tran Thi Dao 2 , Ta Ha Trang 2 , Nguyen Thanh Huyen 2 , Ngo Thi Van Anh 2 , Nguyen Xuan Canh 2

  • 1 Viện Sinh học Nông nghiệp, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Peanut, Sclerotium rolfsii, stem rot disease

    Abstract


    Peanut production has been facing many difficulties due to the increasing incidence of Sclerotinia stem rot disease caused by Sclerotium rolfsiibut there is still no effective solution to deal with this disease. Therefore, this study was conducted to determine the biological characteristics of S. rolfsii, thereby orienting measures tocontrol the disease more effectively. From 10 peanut samples showing stem rot symptoms collected from Thai Binh and Nam Dinh provinces, by inoculating method on PDA medium combined with artificial infection method on peanut plant, 6 fungal strains were determined capable of causing disease, in which LT1 and LN1 expressed the strongest pathogenicity. They were identified as S. rolfsiiisolate LT1 and S. rolfsiistrain LN1, respectively. Two isolates were in the same mycelial compatibility group. Specifically, they grew and formed sclerotia well in PDA medium at 30oC and pH: 5,0-6,0. Simultaneously, on the PDA medium, the colonies of LT1 and LN1 showed maximum growth (>90 mm) after 3 days of culture. Their aerial mycelium was white and abundant. Their sclerotia were formed after 4-5 days of culture and turned brown after 6-7 days with 921 sclerotia (LT1) and 420 sclerotia (LN1). Both of them produced acid to reduce pH of the culture medium sharply and produced extracellular enzymes.

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