Antagonistic Properties of Streptomycessp. VNUA27Strain AgainstFusarium oxysporumCausing Panama Disease on Banana

Received: 04-05-2022

Accepted: 19-08-2022

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Issue: Vol. 20 No. 8 (2022)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Son, D., Mai, N., Thu, N., Hai, N., Dao, T., Anh, N., & Canh, N. (2024). Antagonistic Properties of Streptomycessp. VNUA27Strain AgainstFusarium oxysporumCausing Panama Disease on Banana. Vietnam Journal of Agricultural Sciences, 20(8), 1042–1053. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1032

Antagonistic Properties of Streptomycessp. VNUA27Strain AgainstFusarium oxysporumCausing Panama Disease on Banana

Dinh Truong Son (*) 1 , Nguyen Thi Thanh Mai 2 , Nguyen Thi Thu 1 , Nguyen Thanh Hai 1 , Tran Thi Dao 1 , Ngo Thi Van Anh 1 , Nguyen Xuan Canh 1

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam
  • 2 Trung tâm Sinh học thực nghiệm, Viện Ứng dụng Công nghệ

    • Keywords

    Actinomycetes, Streptomyces, Fusarium oxyspoumf. sp. cubense

    Abstract


    Panama disease or banana Fusarium wiltcaused byFusarium oxyspoumf. sp. cubense (Foc)is one of the most destructive plant diseases. In particular, the strain Foc TR4can infect most banana varieties. Compared with traditional methods, biological measures using microorganisms in disease control are considered strategic measures. In this study, actinomycete strain VNUA27 was isolated and evaluated for its ability to antagonize FocTR4. The effect of culture filtrates of the VNUA27 strain on the growth of hyphae and germination ofFocTR4 fungal spores was evaluated. Based on the morphological, physiological, and biochemical characteristics and sequence analysis of 16S rRNA region, actinomycete strain VNUA27 was assigned as StreptomycesdiastatochromogenesVNUA27. The actinomycete strain VNUA27 exhibited high antifungal activity against Foc TR4 (54.78%). After treatment with the strain VNUA27 culture filtrates, the FocTR4 hyphae became shrunk and deformed. Spore samples of FocTR4 treated with culture filtrates of the strain VNUA27 showed a high percentage of non-germinated spores (82.42% inhibition). In particular, the results revealed the function of KSαgene clusters that contributed to the biosynthesis of active secondary metabolites of the strain VNUA27. This study showed that the actinomycete strain VNUA27 is a potential agent for biological control of Foc TR4 of Fusarium wilt withhigh efficiency.

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