Biological Characterization of Streptomycessp. VNUA30 Strain with Antagonistic Activity Against Pathogenic Fungi CausingDisease on some Plants

Received: 04-05-2022

Accepted: 05-07-2022

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Issue: Vol. 20 No. 7 (2022)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Mai, N., Hai, N., Thu, N., Dao, T., & Canh, N. (2024). Biological Characterization of Streptomycessp. VNUA30 Strain with Antagonistic Activity Against Pathogenic Fungi CausingDisease on some Plants. Vietnam Journal of Agricultural Sciences, 20(7), 954–964. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1023

Biological Characterization of Streptomycessp. VNUA30 Strain with Antagonistic Activity Against Pathogenic Fungi CausingDisease on some Plants

Nguyen Thi Thanh Mai (*) 1 , Nguyen Thanh Hai 2 , Nguyen Thi Thu 2 , Tran Thi Dao 2 , Nguyen Xuan Canh 2

  • 1 Trung tâm Sinh học thực nghiệm, Viện Ứng dụng Công nghệ
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Actinomycetes, Streptomyces, plant pathogenic fungi

    Abstract


    Diseases caused by pathogenic fungi account for 80% of plant diseases resulting in a global crop yieldreduction. The use of chemical fungicides for the control of fungal diseases causes environmental pollution and affects human health. Biocontrol using actinomycetes having antifungal activity was reported to be effective and biosafe. This studyfound that actinomycete strain VNUA30 exhibited a broad-spectrum antifungal activity against five plant pathogenic fungi, i.e. Colletotrichum gloeosporioides, Fusarium oxysporumf.sp. cubense(FocTR4), Corynespora casiicola, Sclerotium rolfsii, and Diaporthe sp. with inhibition rate of 62.50%; 52.58%; 72.22%; 57.77%; and 95.37%, respectively. Biological studies showed that strain VNUA30 was able to produce melanoid pigment, siderophore, H2S and IAA, assimilate citrate, reduce nitrate, liquefy gelatin, degrade urea and produce extracellular enzymes chitinase, cellulase, xylanase, protease, and pectinase. Combining the morphological and biochemical characteristics with the 16S rRNA sequence analysis, this strain was identified as Streptomyces deccanensis VNUA30. The results confirmed that actinomycete strain VNUA30 is a potential strain as bio-control agent for fungal diseases on plants with high efficiency.

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