Received: 01-03-2022
Accepted: 27-05-2022
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Effect of Culture Conditions on Mycelial Growth of Agaricus blazeiMurill Strain Ab-1
,
Tran Thuy Linh
2
,
Nguyen Duy Trinh
1
,
Nguyen Van Giang
3
Keywords
Agaricus blazei, mycelium, nutrient, carbon source, nitrogen source
Abstract
Agaricus blazei Murill is a medicinal mushroom with potent immunomodulatory and antitumor activities. Nutrition and environmental conditions are two main factors affecting the mycelial growth of mushrooms. Therefore, the study was carried out to optimize the culture of Agaricus blazei on a pure cultural medium. The carbon sources (glucose, fructose, maltose, sucrose, and lactose), nitrogen sources (NH4Cl, NH4NO3, (NH4)2SO4, NH4HPO4, peptone, and yeast extract) with initial pH values of 4, 5, 6, 7, 8, 9 and incubation temperatures of 15C, 20C, 25C, 30C, and 35C were tested for mycelial growth of Agaricus blazei mushroom strain Ab-1. The mycelial colony diameter, growth rate, and mycelial density were evaluated with P <0.05. Results indicated that glucose and yeast extracts were the most suitable carbon and nitrogen sources for the mycelial growth of strain Ab-1. pH6 and temperature of 25C were also optimal conditions for the growth of Ab-1 mycelia. The mycelial colony's growth rate and diameter reached 8.14 mm/day and 78.44 mm after 10 days.
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