Biological Characterization of Canine Parvovirus Type 2 IsolatesCausing Canine Parvovirus Enteritis in North Viet Nam

Received: 10-03-2021

Accepted: 21-01-2022

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Issue: Vol. 20 No. 3 (2022)

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NÔNG HỌC

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Lam, T., Lan, N., Tuan, N., Yen, N., Hoa, N., & Luyen, L. (2024). Biological Characterization of Canine Parvovirus Type 2 IsolatesCausing Canine Parvovirus Enteritis in North Viet Nam. Vietnam Journal of Agricultural Sciences, 20(3), 292–299. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/969

Biological Characterization of Canine Parvovirus Type 2 IsolatesCausing Canine Parvovirus Enteritis in North Viet Nam

Truong Quang Lam (*) 1, 2 , Nguyen Thi Lan 2 , Nguyen Anh Tuan 2 , Nguyen Thi Yen 2 , Nguyen Thi Hoa 2 , Le Thi Luyen 2

  • 1 Phòng Thí nghiệm trọng điểm Công nghệ sinh học Thú y, Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Canine parvo virus, Isolation, PCR, Sequencing, biological characteristics

    Abstract


    Canine parvovirus (CPV) is a highly contagious viral disease that commonly causes acute gastrointestinal illness in dog breeds in Vietnam. We isolated and determined genotype and biological characteristics of CPV in the CRFK cell line. Three strains of CPV, VNUA.CPV.HN01, VNUA.CPV.HN04 and VNUA.CPV.HY05 in the CRFK cell line were isolated from 30 clinicalsamples. The phylogenetic analysis revealed that all CPVisolates belonged to the genotype CPV2c. CPV did not cause disease in the first passage but caused cytopathogenic effect in the CRFK cells after 2 to 4 blind passages. Cytopathic effect (CPE) occurred within 24 hours post-infection and the cells were completely destroyed after 60 to 72 hours of infection. CPE viral titer was high, at 107,47TCID50/ml. The growth curve reached the peak at 60 to 72 hours after infection and then decreased gradually.

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