Study on the Culture Conditions for Prodution of Pigments and Monacolin Kfrom Monascus purpureusNBRC 4485

Received: 24-09-2021

Accepted: 21-01-2022

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Issue: Vol. 20 No. 3 (2022)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Thanh, N., Thu, P., Chau, L., Long, B., & Phong, H. (2024). Study on the Culture Conditions for Prodution of Pigments and Monacolin Kfrom Monascus purpureusNBRC 4485. Vietnam Journal of Agricultural Sciences, 20(3), 350–358. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/961

Study on the Culture Conditions for Prodution of Pigments and Monacolin Kfrom Monascus purpureusNBRC 4485

Nguyen Ngoc Thanh (*) 1 , Pham Thi Minh Thu 1 , Luu Minh Chau 1 , Bui Hoang Dang Long 1 , Huynh Xuan Phong 1, 2

  • 1 Viện Nghiên cứu và Phát triển Công nghệ Sinh học, Trường Đại học Cần Thơ
  • 2 Viện Công nghệ Sinh học và Thực phẩm, Trường Đại học Cần Thơ

    • Keywords

    Monacolin K, Monascus purpureus, solid-state fermentation, red pigment, yellow pigment

    Abstract


    This study aimed to determine the suitable culture conditions for the production of pigments and monacolin K from mold Monascus purpureus NBRC 4485. The growth and production of pigments and monacolin K of M. purpureusNBRC 4485 were tested in various substrates including red rice, purple rice, wholegrain white rice, white rice, Japanese rice, corn, and soybean. The appropriate conditions of solid-state fermentation for pigments and monacolin K production from M. purpureusNBRC 4485 were investigated through determination of the following solid-state fermentaion factors: initial moisture content of cultural medium (30, 40, 50, 60, 70% w/v), the density of inoculum mold (104, 105, 106spores/g), and incubation time (8 to 24 days). The results show that the wholegrain white rice was considered as the most appropriate substrate among tested substrates. The highest contents of yellow pigment, red pigment, and monacolin K were obtained at 3,057.8 AU/g, 1,781.0 AU/g, and 1,329.3µg/g, respectively. The most suitable conditions to produce pigments and monacolin K from M. purpureusNBRC 4485 on wholegrain white rice were the moisture content of 40% w/w, mold density of 106spores/g, and incubation time of 22 days. The contents of yellow pigment, red pigment, and monacolin K were 6.750,2 AU/g, 4.960,9 AU/g, and 2.089,3 µg/g, respectively.

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