Received: 11-02-2020
Accepted: 11-08-2020
DOI:
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Evaluation of Agro-Biological Characteristics of some Cassava (Manihot EsculentaCrantz) Materials in Field Conditions and Screening of the Resistance to CMV in Artificial Infection
,
Tran Thi Thanh Ha
1
,
Vu Thi Bich Hanh
1
,
Nguyen Van Ha
1
,
Duong Thi Loan
1
,
Vu Van Liet
1
Keywords
Cassava, cassava mosaic virus CMV, artificial infection
Abstract
This study was conducted to evaluate some agro-biological characteristics of 14 domestic and exotic cassava materials in Gia Lam, Hanoi condition, and to evaluate the resistance of the materials through artificial infection experiments by the transplanting method. The material was grown in the field in a sequential experiment without repeating, tracking some agro-biological characteristics. The materials were havested after 8-11 months. The plant height of the materials ranged from 165.2-183.2cm, there are 8 materials with branching height. Most materials have only 1 stem/cluster, CM15, CM21 had 2 stems/cluster and CM60 had 3 stems/cluster. The number of tubers/tree varies from 4.3-15.8 tubers/tree. The highest weight of fresh tubers was obtained CM15 (5.0 kg/tree) and 2 control varieties (5.26-5.82 kg/tree). Using the tranplanting method, evaluated after 2 weeks, 40 successful transplanted trees of 13 materials were evaluated. Genotyping of these transplanted trees was checked by JSP1/JSP2 and JSP1/JSP3 primers detected the presence of ACMV in plants of 8 materials (CM2, CM3, CM8, CM15, CM20, CM60, KM140 and KM94). Assessing the level of infection after 3 months of transplantation, the group of resistant and highly resistant varieties were identified including CM15, CM16, CM21, CM60, CM88, CM50, CM61 and KM94.
References
Akano O., Dixon O., Mba C., Barrera E. & Fregene M. (2002). Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease. Theor. Appl. Genet. 105: 521-525.
Dellaporta S.L., Wood J. & Hisks J.B. (1983). A plant DNA minipreparation: version II, Plant Mol. Biol. Rep. 1: 19-21.
Fregene M., Okogbenin E., Mba C., Angel F., Suarez M.C., Guitierez J., Chavarriaga P., Roca W., Bonierbale M. & Tohme J. (2001). Genome mapping in cassava improvement: challenges, achievements and opportunities. Euphytica. 120: 159-165.
Hoang Kim, Nguyen Van Bo, Reinhardt H. & Hernan C. (2008). Current Situation of Cassava in Vietnam and the selection of cassava doubled haploid (DH) lines derived from CIAT. Paper presented at “Cassava meeting the challenges of the new millennium” hosted by IPBO- Ghent University, Belgium 21-25 July 2008.
Jerome A.H., Martine Z.T., Hermine B.N. ,Justin S.P., Gilles H.T.C., Sergine E.N. & Joseph M.B.C.A. (2019). Evaluation of resistance to cassava mosaic disease in selected African cassava cultivars using combined molecular and greenhouse grafting tools. Physiological and Molecular Plant Pathology. 105: 47-53.
Okogbenin E., Egesi C.N., Olasanmi B., Ogundapo O., Kahya S., Hurtado P., Marin J., Akinbo A.O., Mba C., Gomez H., Vicente C., Baiyeri S., Uguru M., Ewa F. & Fregene M. (2012). Molecular marker analysis and validation of resistance tocassava mosaic disease in elite cassava cultivars in Nigeria. Crop Sci. 52: 2576-2586.
Rwegasira G.M. & Chrissie M.E.R. (2015). Efficiency of non-vector methods of cassava brown streak virus transmission to susceptible cassava plants, Afr. J. Food Agr. Nut. pp. 1684-5374.
Wagaba H., Beyene G., Trembley C., Alicai T., Fauquet C.M. & Taylor N.J. (2013). Efficient transmission of Cassava brown streak disease viral pathogens by chip bud grafting. BMC Res. Notes. 6: 516.