Study on the Virulence Factors ofVibrio parahaemolyticusCaused Acute Hepatopancreatic Necrosis Disease (AHPND) in Farmed WhiteLegShrimp (Litopenaeus vanamei) in Thua Thien Hue Province

Received: 13-03-2020

Accepted: 24-04-2020

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Issue: Vol. 18 No. 3 (2020)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Phuoc, N., Hong, N., & Chung, N. (2024). Study on the Virulence Factors ofVibrio parahaemolyticusCaused Acute Hepatopancreatic Necrosis Disease (AHPND) in Farmed WhiteLegShrimp (Litopenaeus vanamei) in Thua Thien Hue Province. Vietnam Journal of Agricultural Sciences, 18(3), 202–211. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/651

Study on the Virulence Factors ofVibrio parahaemolyticusCaused Acute Hepatopancreatic Necrosis Disease (AHPND) in Farmed WhiteLegShrimp (Litopenaeus vanamei) in Thua Thien Hue Province

Nguyen Ngoc Phuoc (*) 1 , Nguyen Thi Xuan Hong 1 , Nguyen Cong Chung 2

  • 1 Khoa Thuỷ sản, Trường Đại học Nông Lâm Huế
  • 2 Chi cục thú y vùng 3

    • Keywords

    Vibrio parahaemolyticus, white shrimp, virulence factor, Acute hepatopancreatic nerosis disease, AHPND

    Abstract


    The aim of this study was to identified the virulence factors and the pathogenesis of Vibrio parahaemolyticusin farmed white leg shrimp in Thua Thien Hue province. In this study, 8 isolates of V. parahaemolyticuswas recovered from diseased white leg shrimp that showed typical clinical signs of AHPND such as pale-to-white hepatopancreas (HP), significant atrophy of the HP, softshells, guts with discontinuous, or no contents, black spots or streaks visible within the HP. The bacteria were identified as V. parahaemolyticusby using API 20E kit (BioMerieux). Four of 8 isolates have been detected that caused AHPND by using IQ Plus™ AHPND/EMS Plasmid Kit. Data of this study showed that the production of caseinase, phospholipase, haemolysin and mobility of these isolates were significantly stronger than others. The LD50 of isolate that caused AHPND was 105 cfu/mL. By contrast, experimental shrimp were healthy without any clinical signs and no mortality was observed in the treatment immersed in the isolate lacking toxin genes. This study showed strong relationship between virulence factors and pathogenesis of V. parahaemoliticus caused AHPND in shrimp.

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