Purification of IgY from Egg Yolk and Its Application for Prevention and Treatment of Gumboro Disease in Poultry

Received: 27-12-2019

Accepted: 24-02-2020

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Issue: Vol. 17 No. 12 (2019)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Hang, V., Hoa, L., Ngoc, T., Dang, V., Yen, N., Tam, N., … Phan, L. (2024). Purification of IgY from Egg Yolk and Its Application for Prevention and Treatment of Gumboro Disease in Poultry. Vietnam Journal of Agricultural Sciences, 17(12), 976–985. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/623

Purification of IgY from Egg Yolk and Its Application for Prevention and Treatment of Gumboro Disease in Poultry

Vu Thi Thu Hang (*) 1 , Le Quoc Hoa 1 , Trinh Thi Bich Ngoc 1 , Vu Xuan Dang 1 , Nguyen Thi Yen 1 , Nguyen Van Tam 1 , Nguyen Thi Hoa 1 , Nguyen Thi Lan 1 , Le Van Phan 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Gumboro disease, purified IgY, treatment

    Abstract


    The objective of this study was to purify IgY antibodies from chicken egg yolks and apply them for prevention and treatment of Gumboro disease in chickens. In this study, ISA Brown layers of 16 weeks old were used for hyper-immunization to produce eggs with high antibody titers. The immunization results showed that IBD antibody titer sharply increased, especially after hyper-immunizing. The highest titer in the serum samples was obtained at 8 weeks post first vaccination (S/P value was 2.86 ± 0.068). Similar with sera, titter of IBD antibody also raised in eggs, S/P value of IgY-antibody was 2.56 ± 0.111, S/P value of purified IgY from the appropriate yolk was 2.60 ± 0.052. The antibody titer of purified IgY product was stable at 4C with 0.1% sodium benzoate added. The treatment results of chickens that were experimentally infected with gumboro virus showed that 10/10 chickens previously received IgY antibody and 9/10 chickens were treated with IgY antibody after challenging gumboro virus were survived and recovered while 9/10 chickens in the control group that did not receive IgY antibody before challenging test were died.

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