Isolation and Serotyping of Haemophilus parasuisStrains from Pigs Raised in the Provinces of Thanh Hoa, Hung Yen,andHa Nam of Northern Vietnam

Received: 30-07-2018

Accepted: 05-03-2019

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Issue: Vol. 16 No. 12 (2018)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Lam, T., Lan, N., Hoa, N., & Huyen, N. (2024). Isolation and Serotyping of Haemophilus parasuisStrains from Pigs Raised in the Provinces of Thanh Hoa, Hung Yen,andHa Nam of Northern Vietnam. Vietnam Journal of Agricultural Sciences, 16(12), 1068–1078. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/522

Isolation and Serotyping of Haemophilus parasuisStrains from Pigs Raised in the Provinces of Thanh Hoa, Hung Yen,andHa Nam of Northern Vietnam

Truong Quang Lam (*) 1, 2 , Nguyen Thi Lan 2 , Nguyen Thi Hoa 2 , Nguyen Thi Huyen 2

  • 1 Phòng Thí nghiệm trọng điểm Công nghệ sinh học Thú y, Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Haemophilus parasuis, isolation, PCR, multiplex PCR, serotype

    Abstract


    The aim of this study was to determine the serotypes of Haemophilus parasuisstrains isolated from suspected pig cases of glasser disease. A total of 205 specimens including nasal,trachea, joint fluids, heart and lung collected from 41 suspected pigs inThanh Hoa, Hung Yen and Ha Nam province were used for bacterial isolation and identification. The isolates (22 isolatesin total)ofH. parasuisfrom pigs suspected of being infected withH. parasuisshowedthe major characteristics of small, transparent, and non-hemolytic colonies,gram negative, pleomorphic rod from short to long bacilli,catalase, oxidaseandV factor positive, indole and urease negative, and capable of fermentatingglucose, galactose and fructose, and unable to grow on MacConkey agar. Theseisolates were confirmed by specific PCR asH. parasuis. In the present study, for the first time, we reportedthe application of multiplex PCR assay for serotypedetermination ofH. parasuisisolates from the field in Vietnam.mPCRresults showed variable distributions in the serotypesofH. parasuisisolated from the field. Remarkably,serotypes4, 5 and 1 were the most prevalent with 25%, 25% and15%, respectively, followed by serotypes13 and 2 with 5%, and non-typable serotypewith 20%.

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