Insect ResistanceGene(GNA)Transformationinto Cottonby Agrobacterium tumefaciens

Received: 05-01-2015

Accepted: 22-07-2015

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Issue: Vol. 13 No. 5 (2015)

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KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Tri, P., Duong, H., Duc, L., Loc, P., Truong, M., & Ho, N. (2024). Insect ResistanceGene(GNA)Transformationinto Cottonby Agrobacterium tumefaciens. Vietnam Journal of Agricultural Sciences, 13(5), 747–754. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/219

Insect ResistanceGene(GNA)Transformationinto Cottonby Agrobacterium tumefaciens

Pham Duc Tri (*) 1 , Hoang Van Duong 1 , Le Tan Duc 1 , Phan Tuong Loc 1 , Mai Truong 1 , Nguyen Huu Ho 1

  • 1 Viện Sinh học Nhiệt đới, Viện Hàn lâm Khoa học và Công nghệ Việt Nam

    • Keywords

    Agrobacterium tumafaciens, Cotton Coker 312, genetic transformation, GNA gene

    Abstract


    Hypocotyl explants of cotton (Gossypium hirsutum L.) cv. Coker 312 were transformed with Agrobacterium tumefaciens strain EHA 105 containing the GNA gene under the control of RSs1 promoter and selected on callus initiation medium containing kanamycin. Somatic embryogenesis was promoted in the surviving calli. Plantlets were recovered from putative transgenic embryos. Polymerase Chain Reaction (PCR) and Southern blot analysis were used to confirm the integration of GNA transgene into the plant genome. Western blot result indicated the expression of the GNA gene in transgenic cotton plants.

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