Gene Expresssion of Cytokine and Toll-like Receptor Signaling molecule mRNA in Peripheral Blood Mononuclear Cells (PBMCs) from Pigs Co-infected with Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus

Received: 18-03-2015

Accepted: 18-05-2015

DOI:

Views

0

Downloads

0

Issue: Vol. 13 No. 5 (2015)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Hieu, D., Dien, P., Giang, T., & Chung, W. (2024). Gene Expresssion of Cytokine and Toll-like Receptor Signaling molecule mRNA in Peripheral Blood Mononuclear Cells (PBMCs) from Pigs Co-infected with Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus. Vietnam Journal of Agricultural Sciences, 13(5), 723–730. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/212

Gene Expresssion of Cytokine and Toll-like Receptor Signaling molecule mRNA in Peripheral Blood Mononuclear Cells (PBMCs) from Pigs Co-infected with Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus

Dong Van Hieu (*) 1 , Phan Hong Dien 2 , Tran Thi Huong Giang 1 , Wen Bin Chung 2

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Thú y, Đại học Khoa học và Công nghệ Bình Đông, Đài Loan

    • Keywords

    Co-infection, PCV2, pig, PBMCs, PRRSV

    Abstract


    Field and experimental studies have shown that co-infection of PRRSV and PCV2 in pigs were quite common and thus the interactions between both viruses in the host might be critical to the pathogenesis of the disease. The exact interaction mechanisms are unknown and remained to be elucidated. In this study, the expression of cytokine mRNA as well as Toll like receptor signaling molecules associated with innate immunity in peripheral blood mononuclear cells (PBMCs) from pigs infected with PRRSV and/or PCV2 were investigated. Cytokine mRNA and TLR signaling molecule expression were determined by quantitative real-time polymerase chain reaction. Co-infection of pigs with PRRSV and PCV2 led to up-regulated expression of IL-1β and IL-10 mRNA and down-regulated expression of TNF-α and IL-6, IFN-α and IFN-β in PBMCs of pigs co-infected with PRRSV and PCV2. An increased expression of CD14 mRNA was noticed in pigs co-infected with PRRSV and PCV2. A down-regulated expression of IRF-1 and IRF-3 mRNA might also suppress the expression of IFNs that leads to the enhanced replication of PCV2 in pigs co-infected with PRRSV and PCV2.

    References

    Beura LK, Sarkar SN, Kwon B, Subramaniam S, Jones C, Pattnaik AK, Osorio FA (2010). Porcine reproductive and respiratory syndrome virus nonstructural protein 1beta modulates host innate immune response by antagonizing IRF3 activation. J Virol., 84(3):1574-84.

    Couper KN, Blount DG, and Riley EM (2008). IL-10: the master regulator of immunity to infection. Journal of immunology 180: 5771-5777.

    Darwich L, Balasch M, Plana-Duran J, Segales J, Domingo M, and Mateu E (2003). Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens. J Gen Virol., 84: 3453-3457.

    Darwich L, Segales J, Resendes A, Balasch M, Plana-Duran J, and Mateu E. (2008). Transient correlation between viremia levels and IL-10 expression in pigs subclinically infected with porcine circovirus type 2 (PCV2). Research in veterinary science, 84: 194-198.

    Genini S, Delputte PL, Malinverni R, Cecere M, Stella A, Nauwynck HJ, Giuffra E (2008). Genome-wide transcriptional response of primary alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus. J. Gen. Virol., 89 (10): 2550-2564.

    Gudmundsdottir I and Risatti GR (2009). Infection of porcine alveolar macrophages with recombinant chimeric porcine reproductive and respiratory syndrome virus: effects on cellular gene transcription and virus growth. Virus research, 145: 145-150.

    Krishnan J, Selvarajoo K, Tsuchiya M, Lee G, and Choi S (2007). Toll-like receptor signal transduction. Experimental & molecular medicine, 39: 421-438.

    Machida K, Cheng KT, Sung VM, Levine AM, Foung S, and Lai MM (2006). Hepatitis C virus induces toll-like receptor 4 expression, leading to enhanced production of beta interferon and interleukin-6. Journal of virology, 80: 866-874.

    Matsumoto M, Funami K, Oshiumi H, and Seya T (2004). Toll-like receptor 3: A link between toll-like receptor, interferon and viruses. Microbiol Immunol., 48: 147-154.

    Liu CH, Chaung HC, Chang HL, Peng YT, and Chung WB (2009). Expression of Toll-like receptor mRNA and cytokines in pigs infected with porcine reproductive and respiratory syndrome virus. Veterinary microbiology, 136: 266-276.

    Lombardo E (2008). Toll-like receptor signaling: Involvement in graft transplantation. Abril-Junio 27: 69-77.

    Lunney JK, Fritz ER, Reecy JM, KuharD, Prucnal E, Molina R, Christopher-Hennings J, Zimmerman J, and Rowland RR (2010). Interleukin-8, interleukin-1 beta, and interferon-gamma levels are linked to PRRS virus clearance. Viral Immunol., 23: 127-134.

    Pfaffl MW (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic acids research, 29: e45.

    Rossi D and Gaidano G (2003). Messengers of cell death: apoptotic signaling in health and disease. Haematologica, 88: 212-218.

    Sabat R (2011). IL-10 family of cytokines. Cytokine Growth Factor Rev., 21: 315-324.

    Sang Y, Ross CR, Rowland RR, and Blecha F (2008). Toll-like receptor 3 activation decreases porcine arterivirus infection. Viral Immunol., 21: 303-313.

    Shi KC, Guo X, Ge XN, Liu Q, Yang HC (2010). Cytokine mRNA expression profiles in peripheral blood mononuclear cells from piglets experimentally co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Veterinary Microbiology, 140: 155-160.

    Yoo D, Song C, Sun Y, Du Y, Kim O, Liu HC. (2010). Modulation of host cell responses and evasion strategies for porcine reproductive and respiratory syndrome virus. Virus research, 154: 48-60.