Molecular Characterization of ThePorcine Epidemic Diarrhea Viruses Isolated in Quang Tri, Thai Nguyen, and Thai Binh During 2013 - 2014

Received: 22-07-2015

Accepted: 12-10-2015

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Issue: Vol. 13 No. 7 (2015)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Phan, L., Tien, N., Hang, V., Le, H., & Hien, N. (2024). Molecular Characterization of ThePorcine Epidemic Diarrhea Viruses Isolated in Quang Tri, Thai Nguyen, and Thai Binh During 2013 - 2014. Vietnam Journal of Agricultural Sciences, 13(7), 1089–1100. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1545

Molecular Characterization of ThePorcine Epidemic Diarrhea Viruses Isolated in Quang Tri, Thai Nguyen, and Thai Binh During 2013 - 2014

Le Van Phan (*) 1 , Nguyen Trung Tien 2 , Vu Thi Thu Hang 3 , Huynh Thi My Le 1 , Nguyen Ba Hien 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Trung tâm Kiểm nghiệm Thuốc Thú y Trung Ương, Cục Thú y
  • 3 Công ty Cổ phần Phát triển Công nghệ Nông thôn (RTD)

    • Keywords

    PED, phylogeny, spike gene (S)

    Abstract


    Porcine Epidemic Diarrhea (PED) is a serious and contagious swine disease caused by a virus ofCoronaviridaefamily. PED occurs throughout the year but more common in winter season and over 90% of cases occur in less than 7 day old piglets. In this study, 148 samples of faeces and intestines collected from PED-suspected pigs in the 3 provinces of Quang Tri, Thai Nguyen and Thai Binh during 2013 - 2014 werediagnosed by the RT-PCR method. The diagnostic results showed that 57/148 (38.51%) samples were positive with the PED virus. The nucleotide (nt) and deduced amino acid (aa) analyses based on the partial spike (S) gene sequencing showed that the present PED virus strains were closely related to the reference PED virus strains collected in VietNam previously, sharing 94.8 - 98.6% in nt and 93.5 - 98.8% in aa. The phylogenetic analysis revealed that all the present PED virus strains belonged to the group G1-1.

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