Received: 17-06-2014
Accepted: 15-08-2014
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Study on Invitro Culture of Citrus Epicotyl
,
Tong Van Hai
2
,
Doan Van Lu
3
,
Phạm Thị Dung
2
,
Nguyẽn Xuan Viét
4
Keywords
Citrus; Epicotyl, Benzyl adenine (BA); Naphalene acetic acid (NAA); 1H- indole-3-acetic acid (IAA); in vitroculture; Substrate
Abstract
This study was carriedout to develop a complete process of in vitro culture of epicotyls of Vinh orange and Duong Canh mandarin to serve as materials for genetictransfỏmation. Five experiments were conducted focusing on the influence of seed sterilization conditions onefficiency of producing quality epicotylsand concentration of growth phytohormones on shoot and root formation as well as the effects of the substrates onthe growth of plants. The results showed that treatment of seeds for 15 min by shaking with Johnson 10% was suitable condition for the sterilization. The MS mediumcontained30 g/L sucrose,1,0 mg/L vitamin B5 and 8 g/L agar.The optimal concentration of BA for shoot formation of Vinh oranges was 1,5mg/L and for Duong Canh mandarin was1,0 mg/L. The optimal concentrations of NAA/IAA for the root formation of Vinh organe and Duong Canh mandarin were 0.4 mg/0.4 mg/L and 0.4/0.3 mg/L, respectively. The best of substrates for in vitroplants was smoked rice hullssand with 1: 1iweightratio.
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