Assessment of Genetic Diversity of Bombyx moriBased on Coi Sequences

Received: 02-10-2023

Accepted: 26-01-2024

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Issue: Vol. 22 No. 2 (2024)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Nguyen, T., Nhai, N., Duc, H., Duy, N., Giang, P., Thinh, N., … Duc, N. (2024). Assessment of Genetic Diversity of Bombyx moriBased on Coi Sequences. Vietnam Journal of Agricultural Sciences, 22(2), 236–243. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1261

Assessment of Genetic Diversity of Bombyx moriBased on Coi Sequences

Tran Thi Binh Nguyen (*) 1 , Nguyen Thi Nhai , Ho Viet Duc , Nguyen Duc Duy , Pham Thu Giang , Nguyen Hoang Thinh , Nguyen Thi Nhien , Nguyen Huu Duc

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Bivoltine, multivoltine, COI, genetical diversity, mtDNA

    Abstract


    The main goal of study was to use nucleotide sequencing of the cytochrome c oxidase 1 (COI) gene to assess the genetic diversity of 9 bivoltine breeds and 5 native Vietnamese silkworm breeds. DNA Sequencing was carried out using by chain termination method. Data were analyzed by Bioedit, DNAsp and MEGA X softwareThe results indicated 17 polymorphic nucleotide substitution sites when comparing the COI gene sequence of the 14 silkworm breeds in this study with the sequence AB737913.1 on GenBank. Only one polymorphic nucleotide site appeared when comparing the 14 breeds with each other. These silkworm breeds were divided into 2 haplotypes, with a main concentration in haplotype 1. The genetic classification tree shows that 13 out of the 14 studied silkworm breeds were distributed in branch 2, which is the common branch of Chinese and European silkworm breeds. This outcome provides initial information for breeders to strategically use molecular markers in the conservation, exploitation, and development of the genetic resources of silkworm.

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