Identificationof Oyster Mushroom (Pleurotusspp.) Strains in the South Vietnam based on Morphological Characteristics and ITS Sequencing

Received: 15-08-2023

Accepted: 25-12-2023

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Issue: Vol. 21 No. 12 (2023)

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NÔNG HỌC

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Loc, P., Tram, N., Nhan, L., Hoang, P., Dung, N., & Quyen, H. (2024). Identificationof Oyster Mushroom (Pleurotusspp.) Strains in the South Vietnam based on Morphological Characteristics and ITS Sequencing. Vietnam Journal of Agricultural Sciences, 21(12), 1569–1580. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1228

Identificationof Oyster Mushroom (Pleurotusspp.) Strains in the South Vietnam based on Morphological Characteristics and ITS Sequencing

Pham Van Loc (*) 1, 2 , Ngo Thuy Tram 2, 3 , Le Thanh Nhan 3 , Pham Nguyen Duc Hoang 3 , Nguyen Hoang Dung 4, 2 , Ho Bao Thuy Quyen 5

  • 1 Trường Đại học Công thương TP. Hồ Chí Minh
  • 2 Học viện Khoa học và Công nghệ, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • 3 Viện Công nghệ sinh học ứng dụng
  • 4 Viện Sinh học Nhiệt đới, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • 5 Trường Đại học Mở TP. Hồ Chí Minh

    • Keywords

    Identification, ITS sequence, morphology, oyster mushroom

    Abstract


    Oyster mushrooms (Pleurotusspp.) are one of the most commonly cultivated mushrooms in the world. In Vietnam, Pleurotus mushrooms have been popularly grown, especially in the southern region. The classification of oyster mushrooms is important in conservation and development of genetic resources. One of the primary methods for accurate classification of oyster mushrooms is based on their macroscopic and microscopic characteristics. Besides, classification based on the analysis of consensus sequences was widely applied, with ITS being the most used sequence region in fungal genetics. The objective of this study was to identify 15 oyster mushroom strains collected from the southern region through a combination of morphological characteristic analysis and ITS sequencing. The results showed that 10 strains were identified as P. pulmonarius, while 5 other strains were identified as P. ostreatus. The identification results based on ITS sequences were compatible with the morphological identifications. Following the phylogenetic tree based on ITS sequences, 10 phoenix cultivars belonged to P. pulmonariusclade, supported by a bootstrap value of 88%. The oyster cultivars and blue oyster cultivar belonged to Pleurotus cf. floridanus(P. ostreatus species), supported by a bootstrap value of 80%. The results of this study increased the basic data for identification and conservation purposes.

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