Biological Characteristics of Cassava Witches’ Broom Disease Related to Phytoplasma in Dongnai Province

Received: 04-04-2014

Accepted: 09-06-2014

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Issue: Vol. 12 No. 3 (2014)

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NÔNG HỌC

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Thanh, N., Quan, M., Bon, N., Hy, N., Cuong, H., & Hoat, T. (2024). Biological Characteristics of Cassava Witches’ Broom Disease Related to Phytoplasma in Dongnai Province. Vietnam Journal of Agricultural Sciences, 12(3), 325–333. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/111

Biological Characteristics of Cassava Witches’ Broom Disease Related to Phytoplasma in Dongnai Province

Nguyen Duc Thanh (*) 1 , Mai Van Quan 2 , Ngo Gia Bon 2 , Nguyen Huu Hy 3 , Ha Viet Cuong 4 , Trinh Xuan Hoat 2

  • 1 Nghiên cứu sinh, Trường Đại học Nông nghiệp Hà Nội
  • 2 Viện Bảo vệ thực vật
  • 3 Trung tâm Nghiên cứu thực nghiệm Nông nghiệp Hưng Lộc, Đồng Nai
  • 4 Khoa Nông học, Trường Đại học Nông nghiệp Hà Nội

    • Keywords

    ‘Candidatus Phytoplasma asteris’, light traps, insect bodies

    Abstract


    In Vietnam, cassava witches’ broom is an important disease in cassava production. Phytoplasma, 16SrI group-‘Candidatus Phytoplasma asteris’ is the causal agent associated with cassava witches’ broom disease. In this study, leafhopper species were collected by using light traps. Total DNA was extracted from the insect species and the symptomatic plants. Nested polymerase chain reaction assay was used to detect the presence of phytoplasma. Amplification of a fragment 1100 base pair 16S rDNA gene confirmed that insect bodies were infected by the phytoplasma. Restriction fragment length polymorphism analysis using EcoRI and HaeIII endonucleases revealed the insignificant difference between the test samples. Cassava witches’ broom disease is transmitted by cutting through vegetative propagation using diseased plants under screenhouse or field conditions. Whitefly (Aleurodicus dispersus), mealybug (Paracoccus marginatus) and red mite (Tetranychus urticae) do not transmit phytoplasma although they fed on cassava plant in the field.

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