Analysis of Genetic Diversity Based on Phenotypes and SSR Markers and Evaluationof Drought Tolerance of Waxy Maize Inbred Lines for Developing Hybrid Varieties for Northern Moutainous Provinces

Received: 24-02-2014

Accepted: 10-06-2014

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Issue: Vol. 12 No. 3 (2014)

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NÔNG HỌC

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Thao, L., Anh, N., Tan, T., Tuan, P., & Liet, V. (2024). Analysis of Genetic Diversity Based on Phenotypes and SSR Markers and Evaluationof Drought Tolerance of Waxy Maize Inbred Lines for Developing Hybrid Varieties for Northern Moutainous Provinces. Vietnam Journal of Agricultural Sciences, 12(3), 285–297. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/107

Analysis of Genetic Diversity Based on Phenotypes and SSR Markers and Evaluationof Drought Tolerance of Waxy Maize Inbred Lines for Developing Hybrid Varieties for Northern Moutainous Provinces

Le Thi Minh Thao (*) 1 , Nguyen Thi Anh 2 , Tran Thanh Tan 2 , Pham Quang Tuan 2 , Vu Van Liet 3, 4

  • 1 Trường Cao đẳng Cộng đồng Lào Cai
  • 2 Viện nghiên cứu và Phát triển cây trồng, Trường Đại học Nông nghiệp Hà Nội
  • 3 Viện Nghiên cứu và Phát triển cây trồng, Trường Đại học Nông nghiệp Hà Nội
  • 4 Khoa Nông học, Trường Đại học Nông nghiệp Hà Nội

    • Keywords

    Drought tolerance, genetic diversity, heterotic group, inbred maize line, waxy maize

    Abstract


    Assessing genetic diversity and heterotic grouping of 24 waxy maize inbred lines in S8 to S10 generations were performed using morphological characters and molecular markers (SSR). The field experiment was designed to identify the agronomical characteristics, namely growth duration, plant height, anthesis-silking interval, yield and yield components. Results showed that the maize inbred lines possessed agronomic characters suitable to develop single crosses. Based on genetic diversity analysis of morphometric traits, 24 inbred lines were grouped into six heterotic groups at similarity value of 0.25. Genetic diversity analysis of inbred lines by 19 SSR markers detected 19 loci with a total of 75 alleles and average of 4 alleles per locus. Polymorphic Information Content (PIC) varied from 0.36 to 0.81. At genetic similarity value of 0.83, 24 inbred lines were divided into five groups. Of 24 lines tested, 5 lines, viz. I5, I9, I8, I23, I15 were identified to be drought tolerant. These lines belonged to the different heterotic groups and therefore they can be used for hybrid waxy maize breeding for drought prone environment such as rainfed farming condition for northern moutainous provinces.

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