Received: 19-07-2021
Accepted: 05-07-2022
DOI:
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Isolation, Selection and Identification of Nitrogen Fixing Endophytic Bacteriafrom Ming aralia (Polyscias fruticosaL. Harms)
,
Tran Ngoc Huu
1
,
Nguyen Hong Hue
1
,
Le Vinh Thuc
1
,
Tran Chi Nhan
2
,
Ly Ngoc Thanh Xuan
2
,
Nguyen Khanh Linh
3
,
Nguyen Quoc Khuong
4, 5, 6, 7
Keywords
Endophytic bacteria, Ming aralia, Polyscias fruticosaL. Harms, nitrogen fixation
Abstract
The objective of study was to determine nitrogen fixing endophytic bacteriafrom Polyscias fruticosa(L.) Harms. Thirteen leaves and 11 root samples Ming aralia collected in Tri Ton district, An Giang province were used for isolating nitrogen fixing endophytic bacteria on NFB medium with free nitrogen. Results showed that the 51 isolates were isolated from leaf and root Ming aralia, in which 41 strains possessed the acidic resistance at pH 5.0. Strain LP5-L1showed highest nitrogen fixing activity, with the ammonium content of 35.3 ± 5.24 mg/l. However, strain LP1-R4 was selected for their functions to produce N, P and IAA with 31.6 ± 0.39, 18.8 ± 0.78, and 6.65 ± 0.26 mg/l, respectively. The selected strain was identified as Bacillus circulans LP1-R4 based on 16S rDNA nucleotide sequences. The efficacy of a selected strain should be evaluated for replacing N for Ming aralia in pot and field conditions.
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