Received: 12-10-2021
Accepted: 05-07-2022
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Exploring Prevalenceof Mycoplasma hyorhinisInfection by PCR Method in Pigs Raised in Hung Yen Province
,
Nguyen Thi Thu Huong
3
,
Nguyen Thi Lan
3
,
Dao Le Anh
3
,
Le Thi Trang
3
,
Vu Thi Anh
3
Keywords
M. hyorhinisinfection in pigs, prevalence, PCR, Hung Yen province
Abstract
The aim of this study was to determine the infection rate of Mycoplasma hyorhinisfrom suspected pigs in Khoai Chau, Van Giang, and Yen My districts of Hung Yen province.A total of 52 clinical samples of suspected pigs were used to determine the infection rate of M. hyorhinisby using the PCR method. The results showed that the infection rate of M. hyorhinisin 3 districts in Hung Yen province was relatively high at 26.92%, in which infection rate was recorded in Khoai Chau with 31.82%, Van Giang 25.00% and Yen My 21.43%. The rate of infection with M. hyorhinisin pigs at 5-10 weeks of age was the highest at 29.41% and was similar to pigs >10 weeks old 28.57%,while pigs <5 weeks oldshowed lowest infection rate 21.43%. Research also determined that the rate of co-infection of M. hyorhiniswith M. hyopneumoniaeaccounted for 28.57%, H. parasuis35.71% and S. suis21.43%. Comparison of macroscopic findings and PCR results showed that pigs infected with M. hyorhinisexhibited the main pathological feature with a severe fibrinous pericarditis.
References
Abhijit K. Barate, Hwi-Young Lee, Hye-Won Jeong, Lam Quang Truong, Hong-Gu Joo&Tae-Wook Hahn (2012). An improved multiplex PCR for diagnosis and differentiation of Mycoplasma hyopneumoniaeand Mycoplasma hyorhinis. Korean Journal of Veterinary Research.52(1):39-43.
Boetner A.G., Binder M. & Bille-Hansen V. (1987). Streptococcus suis infections in Danish pigs and experimental infection with Streptococcus suis serotype 7. Acta Pathol Microbiol Immunol Scand B. 95: 233-239.
Caron J., Ouardani M. & Dea S. (2000). Diagnosis and differentiation of Mycoplasma hyopneumoniaeand Mycoplasma hyorhinisinfections in pigs by PCR amplification of the p36 and p46 genes. J Clin Microbiol. 38: 1390-1396.
Gois M., Kuksa F. & Sisak F. (1977). Experimental infection of gnotobiotic piglets with Mycoplasma hyorhinisand Bordetella bronchiseptica.Zentralbl Veterinarmed. B24: 89-96.
Huỳnh Thị Mỹ Lệ, Tạ Thị Kim Chung, Vũ Thị Ngọc, Cao Thị Bích Phượng & Chu Thị Thanh Hương (2018). Ứng dụng phản ứng Multiplex Nested PCR để phát hiện Haemophilus parasuis, Mycoplasma hyorhinisvà Streptococcus suisgây bệnh viêm đa thanh mạc ở lợn. Kỷ yếu Hội thảo khoa học nữ cán bộ viên chức. Nhà xuất bản Học viện Nông nghiệp. tr. 167-175.
Kang I., Kim D., Han K., Seo H.W., Oh Y., Park C., Lee J., Gottschalk M. & Chae C. (2012). Optimized protocol for multiplex nested polymerase chain reaction to detect and differentiate Haemophilus parasuis, Streptococcus suis, and Mycoplasma hyorhinisin formalin-fixed, paraffin-embedded tissues from pigs with polyserositis. Canadian Journal of Veterinary Research. 76: 195-200.
Lee J.A., Oh Y.R., Hwang M.A., Lee J.B., Park S.Y., Song C.S. & Lee S.W. (2016). Mycoplasma hyorhinisis a potential pathogen of porcine respiratory disease complex that aggravates pneumonia caused by porcine reproductive and respiratory syndrome virus. Veterinary Immunology and Immunopathology. 177: 48-51.
Lin J.H., Chen S.P., Yeh K.S. & Weng C.N. (2006).Mycoplasma hyorhinis in Taiwan: Diagnosis and isolation of swine pneumonia pathogen. Vet Microbiol. 115: 111-116.
Lobo E., Poveda C., Gupta R., Suarez A., Hernández Y., Ramírez A. & Poveda J.B. (2011). Mycoplasmas hyorhinisin different regions of Cuba. Diagnosis. Braz J Microbiol. 42: 721-725.
Luehrs A., Siegenthaler S., Grützner N., Grosse Beilage E., Kuhnert P. & Nathues H. (2017). Occurrence of Mycoplasma hyorhinisinfections in fattening pigs and association with clinical signs and pathological lesions of Enzootic Pneumonia.Vet Microbiol.203: 1-5.
Miranda-MoralesR.E., TrejoV.R., López-CerinoL.E., Carrillo-Casas E.M., Sarmiento-SilvaR.E.,Trujillo-Ortega M.E., Figueroa R.B. & Trigo-TaveraF.J. (2020). Frequency of M. hyopneumoniae, M. hyorhinisand M. hyosynoviaein nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia. Revista Mexicana de Ciencias Pecuarias. 11(4): 946-960.
Morita T., Ohiwa S., Shimada A., Kazama S., Yagihashi T. & Umemura T. (1999). Intranasally inoculatedMycoplasma hyorhinis causes eustachitis in pigs. Vet 82 Pathol. 36:174-178.
Morita T., Sasaki A., Kaji N., Shimada A., Kazama S., Yagihashi T., Umemura T. (1998). Induction of temporary otitis media in specific-pathogen-free pigs by intratympanic inoculation ofMycoplasma hyorhinis.Am J Vet Res. 59: 869-873.
Okwumabua O., O'Connor M. & Shull E. (2003). A polymerase chain reaction (PCR) assay specific for Streptococcus suisbased on the gene encoding the glutamate dehydrogenase. FEMS Microbiol Lett. 218(1): 79-84.
Oliveira S., Galina L. & Pijoan C. (2001). Development of a PCR test to diagnose Haemophilus parasuisinfections. J Vet. Diagn Invest. 13(6): 495-501.
Pallarés F.J., Halbur P.G., Schmitt C.S., Roth J.A., Opriessnig T., Thomas P.J., Kinyon J.M., Murphy D., Frank D.E. & Hoffman L.J. (2003). Comparison of experimental models for Streptococcus suisinfection of conventional pigs. Canadian Journal of Veterinary Research.67: 225-228.
Riley M.G., Russell E.G. & Callinan R.B. (1977). Haemophilus parasuis infection in swine. J Am Vet Med Assoc. 171(7):649-51.