Ngày nhận bài: 01-06-2020 / Ngày duyệt đăng: 10-01-2022
Pituitary adenylate cyclase-activating polypeptide is abundantly expressed in different hypothalamic nuclei that centrally regulate appetite. We have also recently revealed that the neuropeptide pituitary adenylate cyclase-activating polypeptideincreased appetite in mice. To further elucidate the role of pituitary adenylate cyclase-activating polypeptideneurons, this study utilized a biochemical approach with designer receptors exclusively activated by designer drugssystem and examined the synaptic connectivity of neurons by synapsin expression vectors. We demonstrated that the selective activation or suppression of pituitary adenylate cyclase-activating polypeptideneurons by designer receptors exclusively activated by designer drugssystemin the ventromedial hypothalamus significantly increased or decreased food intake, respectively. In addition, this study indicated a connection between pituitary adenylate cyclase-activating polypeptideneurons in the ventromedial hypothalamuswith agouti-related peptideneurons in the Arc region. These results suggested that food intake in mice is triggered by the increase in pituitary adenylate cyclase-activating polypeptideexpression in the ventromedial hypothalamusvia modulation of agouti-related peptideexpression, pointing to Pituitary adenylate cyclase-activating polypeptideor agouti-related peptideinhibition as a clinically important therapeutic strategy against obesity in the future.
